SPAG6 silencing induces apoptosis in the myelodysplastic syndrome cell line SKM‑1 via the PTEN/PI3K/AKT signaling pathway in vitro and in vivo

International Journal of Oncology
Jiaxiu YinLin Liu

Abstract

Apoptosis is a multi-step mechanism of cell self‑destruction for maintaining cellular homeostatic balance. Accumulating evidence indicates that abnormal apoptosis promotes the evolution and progression of myelodysplastic syndromes (MDS). As a novel cancer-testis antigen, sperm‑associated antigen 6 (SPAG6) has been reported to regulate apoptosis through the tumor necrosis factor-related apoptosis-inducing ligand signaling pathway in the MDS cell line SKM‑1. However, the mechanism of the intrinsic cell death pathway for apoptosis induction by SPAG6 silencing is unclear. In the present study, the in vitro effects of SPAG6 silencing were investigated in SKM‑1 cells through extensive biochemical and molecular approaches. Western blotting and reverse transcription-quantitative polymerase chain reaction were used to detect the expression of SPAG6 and activation of PTEN/PI3K/AKT signal pathway. Additionally, SKM‑1 cells transduced with SPAG6 short hairpin RNA (shRNA) lentivirus were treated with the phosphatidylionositol 3-kinase (PI3K) inhibitor LY294002 or pan caspase inhibitor z‑VAD‑fmk and the apoptosis rates were measured by flow cytometry, and the expressions of associated proteins were examined by western blot analysis. A mouse ...Continue Reading

References

Dec 30, 1999·Nature Genetics·F FuksT Kouzarides
Feb 29, 2000·Cell·A Di Cristofano, P P Pandolfi
Jan 12, 2001·Molecular and Cellular Biology·A H KimM V Chao
Jun 28, 2001·Leukemia & Lymphoma·J E Parker, G J Mufti
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 5, 2002·Nature·Ina RheeBert Vogelstein
May 11, 2002·Nature Reviews. Cancer·Frederik H Igney, Peter H Krammer
Jul 12, 2002·Current Gene Therapy·Michaela Scherr, Matthias Eder
Aug 9, 2002·Molecular and Cellular Biology·Rossana SapiroJerome F Strauss III
Mar 19, 2003·European Journal of Clinical Investigation·T SchöndorfM Hoopmann
Nov 5, 2004·Cellular and Molecular Life Sciences : CMLS·A HermannA Jeltsch
Apr 28, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daniel SteinbachBernd Gruhn
Jul 1, 2006·American Journal of Respiratory Cell and Molecular Biology·Kim M LonerganWan L Lam
Nov 3, 2007·Experimental Hematology·Daniella B Kerbauy, H Joachim Deeg
Feb 15, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Julie SchanzDetlef Haase
Apr 5, 2012·Nature Reviews. Molecular Cell Biology·Min Sup SongPier Paolo Pandolfi
Dec 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daniel SteinbachBernd Gruhn
Aug 28, 2016·Journal of Molecular Histology·Xinde HuShanting Zhao
Jan 4, 2017·Journal of the National Comprehensive Cancer Network : JNCCN·Peter L GreenbergCourtney Smith
Jan 18, 2017·Molecular Cancer·Corinna AltenbergerSabine Zöchbauer-Müller
May 27, 2017·Molecular Genetics & Genomic Medicine·Dana M BisStephan Züchner

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Citations

Jan 10, 2019·Marine Drugs·Geert van WeeldenJohanna M A Pijnenborg
May 16, 2020·Bioscience Reports·Xudong WangJianping Yang
Mar 27, 2019·Journal of Molecular Histology·Jimena AlciaturiRossana Sapiro
May 25, 2021·Disease Models & Mechanisms·Lauren G FalkenbergJoshua S Waxman

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