SpAHA1 and SpSOS1 Coordinate in Transgenic Yeast to Improve Salt Tolerance

PloS One
Yang ZhouXingyu Jiang

Abstract

In plant cells, the plasma membrane Na+/H+ antiporter SOS1 (salt overly sensitive 1) mediates Na+ extrusion using the proton gradient generated by plasma membrane H+-ATPases, and these two proteins are key plant halotolerance factors. In the present study, two genes from Sesuvium portulacastrum, encoding plasma membrane Na+/H+ antiporter (SpSOS1) and H+-ATPase (SpAHA1), were cloned. Localization of each protein was studied in tobacco cells, and their functions were analyzed in yeast cells. Both SpSOS1 and SpAHA1 are plasma membrane-bound proteins. Real-time polymerase chain reaction (PCR) analyses showed that SpSOS1 and SpAHA1 were induced by salinity, and their expression patterns in roots under salinity were similar. Compared with untransformed yeast cells, SpSOS1 increased the salt tolerance of transgenic yeast by decreasing the Na+ content. The Na+/H+ exchange activity at plasma membrane vesicles was higher in SpSOS1-transgenic yeast than in the untransformed strain. No change was observed in the salt tolerance of yeast cells expressing SpAHA1 alone; however, in yeast transformed with both SpSOS1 and SpAHA1, SpAHA1 generated an increased proton gradient that stimulated the Na+/H+ exchange activity of SpSOS1. In this scenari...Continue Reading

References

Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·R A GaxiolaG R Fink
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·R A GaxiolaG R Fink
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Quan-Sheng QiuJian-Kang Zhu
Jun 13, 2002·Physiologia Plantarum·Loubna KerkebMaría Pilar Rodríguez-Rosales
Jun 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Francisco J QuinteroJose M Pardo
Mar 4, 2006·Journal of Experimental Botany·José M PardoFrancisco J Quintero
Dec 5, 2006·Plant Physiology·Juliana Martínez-AtienzaFrancisco J Quintero
Sep 11, 2007·The Plant Journal : for Cell and Molecular Biology·Beom-Gi KimSheng Luan
Mar 5, 2008·Archives of Biochemistry and Biophysics·Haixia XuDangqun Cui
Jun 21, 2008·The New Phytologist·Timothy J Flowers, Timothy D Colmer
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Rainer WaadtJörg Kudla
Jul 3, 2009·Plant Physiology·Dong-Ha OhHans J Bohnert
Oct 2, 2009·Plant Signaling & Behavior·M Pilar Rodríguez-RosalesKees Venema
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Francisco J QuinteroJose M Pardo
Jul 23, 2013·Archives of Biochemistry and Biophysics·Sha HongXingyu Jiang

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Citations

Dec 21, 2019·Journal of Experimental Botany·Xiaochang YinXingyu Jiang
May 6, 2019·International Journal of Molecular Sciences·Antonella LocascioLynne Yenush
Nov 8, 2018·International Journal of Molecular Sciences·Mei ZhangShu-Guang Jian
Jul 15, 2021·Physiologia Plantarum·Ankur Singh, Aryadeep Roychoudhury

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Datasets Mentioned

BETA
JX674067
DQ925817
JX628604

Methods Mentioned

BETA
transgenic
PCR
salt treatment
confocal microscopy

Software Mentioned

SDS plate utility
TMHMM
MEGA5

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