SPARC-Dependent Cardiomyopathy in Drosophila

Circulation. Cardiovascular Genetics
Paul S HartleyKaren Ocorr

Abstract

The Drosophila heart is an important model for studying the genetics underpinning mammalian cardiac function. The system comprises contractile cardiomyocytes, adjacent to which are pairs of highly endocytic pericardial nephrocytes that modulate cardiac function by uncharacterized mechanisms. Identifying these mechanisms and the molecules involved is important because they may be relevant to human cardiac physiology. This work aimed to identify circulating cardiomodulatory factors of potential relevance to humans using the Drosophila nephrocyte-cardiomyocyte system. A Kruppel-like factor 15 (dKlf15) loss-of-function strategy was used to ablate nephrocytes and then heart function and the hemolymph proteome were analyzed. Ablation of nephrocytes led to a severe cardiomyopathy characterized by a lengthening of diastolic interval. Rendering adult nephrocytes dysfunctional by disrupting their endocytic function or temporally conditional knockdown of dKlf15 led to a similar cardiomyopathy. Proteomics revealed that nephrocytes regulate the circulating levels of many secreted proteins, the most notable of which was the evolutionarily conserved matricellular protein Secreted Protein Acidic and Rich in Cysteine (SPARC), a protein involved...Continue Reading

References

Apr 11, 2001·The Journal of Biological Chemistry·S Tartare-DeckertE Van Obberghen
Sep 27, 2002·Genesis : the Journal of Genetics and Development·Deborah A KimbrellKathy Beckingham
Feb 19, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sean E McGuireRonald L Davis
Nov 27, 2004·Nature Genetics·Robert J WessellsRolf Bodmer
Apr 29, 2005·Development·Seung-Hye JungUtpal Banerjee
May 6, 2005·Nature·Daniela PanákováSuzanne Eaton
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Karen OcorrRolf Bodmer
Mar 18, 2008·Nature Methods·Sung Kyu ParkJohn R Yates
Mar 22, 2008·Experimental Cell Research·Debjani DasManeesha Inamdar
Dec 24, 2008·The Journal of Experimental Medicine·Mark W M SchellingsStephane Heymans
Feb 24, 2009·Developmental Biology·Tina BuechlingRolf Bodmer
Mar 11, 2009·Methods : a Companion to Methods in Enzymology·Laurence M BrillDieter A Wolf
Jun 27, 2009·PLoS Genetics·Sandra Fausia SoukupDavid Gubb
Mar 3, 2010·Nature Reviews. Endocrinology·Katarina Kos, John P H Wilding
Jul 6, 2010·Biochimica Et Biophysica Acta·Svetlana N RadyukWilliam C Orr
Aug 16, 2011·Developmental Cell·José Carlos Pastor-Pareja, Tian Xu
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Dec 30, 2011·Nature Methods·Florian HalbritterSimon R Tomlinson
Jan 18, 2012·The International Journal of Biochemistry & Cell Biology·Amy D Bradshaw
Apr 27, 2012·Physiological Reviews·Nikolaos G Frangogiannis
Jul 18, 2012·The Open Rheumatology Journal·Jessica Trombetta-Esilva, Amy D Bradshaw
Dec 25, 2012·Journal of the American Society of Nephrology : JASN·Fujian ZhangZhe Han
Jan 18, 2013·PLoS Genetics·Jianbo NaRoss Cagan
Jul 11, 2013·PloS One·Björn HandkeChristian F Lehner
Nov 14, 2013·Circulation Research·Meera Cozhimuttam ViswanathanAnthony Cammarato
Mar 25, 2014·Cell Reports·Hui-Ying LimRolf Bodmer
May 2, 2014·BioMed Research International·Lisandra E de Castro BrásAmy D Bradshaw
Jul 21, 2015·Cell Reports·Jianbo NaRoss L Cagan

❮ Previous
Next ❯

Citations

Apr 13, 2017·Cell and Tissue Research·Martin Helmstädter, Matias Simons
Jan 31, 2018·Journal of Cellular Biochemistry·Florian HochapfelMichael P Krahn
Apr 21, 2016·Circulation. Cardiovascular Genetics·Matthew J Wolf
Dec 23, 2017·Frontiers in Pediatrics·Martin HelmstädterTobias Hermle
Dec 27, 2016·World Journal of Gastroenterology : WJG·Jing-Ran SuYan-Qing Li
Dec 19, 2018·Journal of Cardiovascular Translational Research·Shu ZhouYan Wang
Mar 3, 2021·Cellular and Molecular Life Sciences : CMLS·Stefanie HeidenMichael P Krahn
Apr 3, 2021·Journal of the American Society of Nephrology : JASN·Michael Mysh, John S Poulton
Jul 29, 2021·Scientific Reports·Estela Selma-SorianoJosep Redón

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

SignalP
Sorcerer Enterprise
SEQUEST Tryptic
ImageJ
Micro
QTools
GeneProf
Protein prophet
Manager
Peptide

Related Concepts

Related Feeds

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.