SPARC enables genetic manipulation of precise proportions of cells.

Nature Neuroscience
Jesse Isaacman-BeckThomas R Clandinin

Abstract

Many experimental approaches rely on controlling gene expression in select subsets of cells within an individual animal. However, reproducibly targeting transgene expression to specific fractions of a genetically defined cell type is challenging. We developed Sparse Predictive Activity through Recombinase Competition (SPARC), a generalizable toolkit that can express any effector in precise proportions of post-mitotic cells in Drosophila. Using this approach, we demonstrate targeted expression of many effectors in several cell types and apply these tools to calcium imaging of individual neurons and optogenetic manipulation of sparse cell populations in vivo.

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Methods Mentioned

BETA
dissection
transgenic
PCR
restriction digest
dissections

Software Mentioned

MADM
Flybow
FlpOut
SPARC
Brainbow
Adobe Photoshop
Gal4
SPARC2
Addgene
Imaris

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