Apr 12, 2020

PALP: An imaging method for detecting and quantifying polyunsaturated phospholipids via peroxidation

BioRxiv : the Preprint Server for Biology
Yilong ZouS. L. Schreiber

Abstract

Polyunsaturated phospholipids are essential for multiple cellular functions; however, their uncontrolled peroxidation leads to ferroptosis. Here we describe photochemical activation of membrane lipid peroxidation (PALP), which uses localized laser pulses to induce lipid peroxidation photochemically. While PALP bypasses enzymatic requirements for lipid peroxidation, the resulting BODIPY-C11-based signal is largely correlated with local polyunsaturated phospholipid concentration on membranes. This technique enables non-invasive reporting of lipid unsaturation levels and sensitivity to ferroptosis in live cells.

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Mentioned in this Paper

In Vivo
Biochemical Pathway
SPARC
Cellular Process
SPARC protein, human
Extracellular Matrix Assembly
Extracellular
Membrane
Caenorhabditis elegans
Transendothelial Migration

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