Apr 3, 2007

Spare quinones in the QB cavity of crystallized photosystem II from Thermosynechococcus elongatus

Biochimica Et Biophysica Acta
Roland KrivanekMichael Haumann

Abstract

The recent crystallographic structure at 3.0 A resolution of PSII from Thermosynechococcus elongatus has revealed a cavity in the protein which connects the membrane phase to the binding pocket of the secondary plastoquinone Q(B). The cavity may serve as a quinone diffusion pathway. By fluorescence methods, electron transfer at the donor and acceptor sides was investigated in the same membrane-free PSII core particle preparation from T. elongatus prior to and after crystallization; PSII membrane fragments from spinach were studied as a reference. The data suggest selective enrichment of those PSII centers in the crystal that are intact with respect to O(2) evolution at the manganese-calcium complex of water oxidation and with respect to the integrity of the quinone binding site. One and more functional quinone molecules (per PSII monomer) besides of Q(A) and Q(B) were found in the crystallized PSII. We propose that the extra quinones are located in the Q(B) cavity and serve as a PSII intrinsic pool of electron acceptors.

  • References31
  • Citations20

References

  • References31
  • Citations20

Citations

Mentioned in this Paper

Spinach preparation
Biochemical Pathway
Manganese
Photopsia
Calcium [EPC]
Crystal - Body Material
Benzoquinone
Dioxygen
Tissue Membrane
Energy Transfer

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