Sparsely cross-linked "nanogel" matrixes as fluid, mechanically stabilized polymer networks for high-throughput microchannel DNA sequencing

Analytical Chemistry
E A DohertyAnnelise E Barron

Abstract

We have developed sparsely cross-linked "nanogels", subcolloidal polymer structures composed of covalently linked, linear polyacrylamide chains, as novel replaceable DNA sequencing matrixes for capillary and microchip electrophoresis. Nanogels were synthesized via inverse emulsion (water-in-oil) copolymerization of acrylamide and a low percentage (approximately 10(-4) mol %) of N,N-methylene bisacrylamide (Bis). Nanogels and nanogel networks were characterized by multiangle laser light scattering and rheometry, respectively, and tested for DNA sequencing in both capillaries and chips with four-color LIF detection. Typical nanogels have an average radius of approximately 230 nm, with approximately 75% of chains incorporating a Bis cross-linker. The properties and performance of nanogel matrixes are compared here to those of a linear polyacrylamide (LPA) network, matched for both polymer weight-average molar mass (M(w)) and the extent of interchain entanglements (c/c). At sequencing concentrations, the two matrixes have similar flow characteristics, important for capillary and microchip loading. However, because of the physical network stability provided by the internally cross-linked structure of the nanogels, substantially long...Continue Reading

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Citations

May 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert G BlazejRichard A Mathies
Aug 29, 2007·Analytica Chimica Acta·Lin He, Chee-Seng Toh
Nov 12, 2005·Angewandte Chemie·Satish Nayak, L Andrew Lyon
Nov 27, 2004·Electrophoresis·Cheuk-Wai KanAnnelise E Barron

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