Spasticity Treatment Ameliorates the Efficacy of Melatonin Therapy in Experimental Autoimmune Encephalomyelitis (EAE) Mouse Model of Multiple Sclerosis.

Cellular and Molecular Neurobiology
Majid GhareghaniNaser Farhadi

Abstract

Multiple sclerosis (MS) is a progressive inflammatory demyelinating disease in the central nervous system (CNS). Melatonin is an effective treatment in MS patients and experimental autoimmune encephalomyelitis (EAE), a mouse model of MS. Melatonin secretion peaks at 2 AM, concomitant with the time at which the muscles are resting and the body is exerting its antioxidant activity. The current study was designed to investigate combination treatment of baclofen, a muscle relaxant drug, and melatonin in EAE mice. Results showed that melatonin (Mel) alone or in combination with baclofen (Bac + Mel) reduced clinical scores and demyelination by significantly increasing myelin oligodendrocyte glycoprotein (MOG) levels, a marker for mature oligodendrocytes, compared to EAE mice. Moreover, Mel or Bac + Mel therapy caused a significant increase in IL-4 serum levels, an anti-inflammatory cytokine, whereas IFN-γ serum levels, a pro-inflammatory cytokine, were significantly reduced. On the other hand, Mel or Bac + Mel caused a significant reduction in malondialdehyde (MDA) levels, a marker of oxidative stress, in comparison to EAE mice. In contrast, the activity of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) was signifi...Continue Reading

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Citations

Dec 18, 2019·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Charalampos Skarlis, Maria Anagnostouli
May 6, 2019·Clinical Drug Investigation·Mahshid Yeganeh SalehpourMohammad Hosein Farzaei
Oct 3, 2020·Experimental Neurology·Frederique WietersMarkus Aswendt
May 14, 2021·Frontiers in Pharmacology·Monika Dmitrzak-WeglarzJoanna Pawlak
May 29, 2021·Translational Neuroscience·Majid GhareghaniKazem Zibara

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Methods Mentioned

BETA
Enzyme-Linked Immunosorbent Assay
protein assay
MDA

Software Mentioned

LabWorks
GraphPad Prism

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