SPATC1L maintains the integrity of the sperm head-tail junction

EMBO Reports
Jihye KimChunghee Cho

Abstract

Spermatogenesis is a tightly regulated process involving germ cell-specific and germ cell-predominant genes. Here we investigate a novel germ cell-specific gene, Spatc1l (spermatogenesis and centriole associated 1 like). Expression analyses show that SPATC1L is expressed in mouse and human testes. We find that mouse SPATC1L localizes to the neck region in testicular sperm. Moreover, SPATC1L associates with the regulatory subunit of protein kinase A (PKA). Using CRISPR/Cas9-mediated genome engineering, we generate mice lacking SPATC1L. Disruption of Spatc1l in mice leads to male sterility owing to separation of sperm heads from tails. The lack of SPATC1L is associated with a reduction in PKA activity in testicular sperm, and we identify capping protein muscle Z-line beta as a candidate target of phosphorylation by PKA in testis. Taken together, our results implicate the SPATC1L-PKA complex in maintaining the stability of the sperm head-tail junction, thereby revealing a new molecular basis for sperm head-tail integrity.

References

Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A R BellvéD A O'Brien
Mar 1, 1994·Archives of Histology and Cytology·M HamasakiH Matsuo
Jan 15, 1996·FEBS Letters·V T NachmiasE Barron-Casella
Jan 1, 1995·Reproduction, Fertility, and Development·E M Eddy
Nov 14, 1998·Seminars in Cell & Developmental Biology·E M Eddy
Apr 13, 2000·International Journal of Andrology·Y ToyamaS Yuasa
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·J L DesseynG S McKnight
Aug 3, 2000·Frontiers in Bioscience : a Journal and Virtual Library·B S Skalhegg, K Tasken
May 23, 2002·Recent Progress in Hormone Research·Edward M Eddy
Jul 13, 2004·Trends in Cell Biology·Ville O PaavilainenPekka Lappalainen
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael A NolanG Stanley McKnight
Sep 15, 2004·Trends in Biochemical Sciences·Martin A Wear, John A Cooper
Sep 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mark VeugelersCraig T Basson
Oct 30, 2004·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·D PanidisM Mamopoulos
Jun 30, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Katerina DvorákováJirí Palecek
Feb 10, 2006·International Journal of Andrology·Niels E SkakkebaekJorma Toppari
May 27, 2006·Molecular Endocrinology·Kimberly A BurtonG Stanley McKnight
Feb 10, 2007·Physiology·Kimberly A Burton, G Stanley McKnight
Jun 12, 2008·International Review of Cell and Molecular Biology·John A Cooper, David Sept
Jul 26, 2008·Reproductive Biology and Endocrinology : RB&E·Namhoe BaekChunghee Cho
Sep 23, 2008·Genes & Development·Rebecca C FryLeona D Samson
Nov 7, 2009·PLoS Genetics·Keizo TokuhiroHiromitsu Tanaka
Feb 13, 2010·Journal of Molecular Endocrinology·Guillaume Pidoux, Kjetil Taskén
Apr 27, 2010·Journal of Proteome Research·Jacek R WiśniewskiMatthias Mann
Jun 15, 2010·Human Reproduction·M GotoE M Eddy
Aug 15, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Kefei YangSigrid Hoyer-Fender
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Shuiqiao YuanWei Yan
Dec 3, 2016·American Journal of Human Genetics·Fuxi ZhuYunxia Cao

❮ Previous
Next ❯

Citations

Jun 4, 2019·Genome Biology·Chathura J GunasekaraRobert A Waterland
May 12, 2020·Molecular Human Reproduction·Yi-Ru ShenPao-Lin Kuo
Jan 17, 2021·Journal of Assisted Reproduction and Genetics·Marziyeh Mazaheri MoghaddamEbrahim Sakhinia
Aug 19, 2020·Molecular and Cellular Endocrinology·Tomer Avidor-ReissEmily Lillian Fishman
Jul 17, 2021·Frontiers in Cell and Developmental Biology·Yunfei ZhangXiaowei Xing
Aug 21, 2019·Journal of Proteome Research·Charles PineauCecilia Lindskog
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Yanwei ShaXiaoli Wei
Dec 23, 2021·Biomédica : revista del Instituto Nacional de Salud·Omid JazayeriFereshteh Mir Moammadrezaei

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.