Spatial analysis of lipid metabolites and expressed genes reveals tissue-specific heterogeneity of lipid metabolism in high- and low-oil Brassica napus L. seeds

The Plant Journal : for Cell and Molecular Biology
Shaoping LuLiang Guo

Abstract

Despite the importance of oilseeds to worldwide human nutrition, and more recently to the production of bio-based diesel fuels, the detailed mechanisms regulating seed oil biosynthesis remain only partly understood, especially from a tissue-specific perspective. Here, we investigated the spatial distributions of lipid metabolites and transcripts involved in oil biosynthesis from seeds of two low-erucic acid genotypes of Brassica napus with high and low seed-oil content. Integrated results from matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) of lipids in situ, lipidome profiling of extracts from seed tissues, and tissue-specific transcriptome analysis revealed complex spatial distribution patterns of lipids and transcripts. In general, it appeared that many triacylglycerol and phosphatidylcholine species distributed heterogeneously throughout the embryos. Tissue-specific transcriptome analysis identified key genes involved in de novo fatty acid biosynthesis in plastid, triacylglycerols assembly and lipid droplet packaging in the endoplasmic reticulum (ER) that may contribute to the high or low oil phenotype and heterogeneity of lipid distribution. Our results imply that transcriptional regulatio...Continue Reading

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Citations

Jun 7, 2018·The Plant Journal : for Cell and Molecular Biology·Sheila McCormick
Feb 23, 2020·Frontiers in Plant Science·Shaoping LuLiang Guo
May 8, 2020·Foods·Yoshimura Yukihiro, Nobuhiro Zaima
Feb 11, 2021·International Journal of Molecular Sciences·Romane MiraySébastien Baud
Oct 14, 2020·The Plant Journal : for Cell and Molecular Biology·Shan TangLiang Guo
Jul 9, 2020·Progress in Lipid Research·Sandra M CorreaYariv Brotman

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