Abstract
The ideal trachea replacement would be a living graft that is genetically identical to the host, avoiding the need for immunosuppression. We have developed a mouse model of syngeneic tracheal transplant that results in long-term survival without graft stenosis or delayed healing. To understand how host cells contribute to tracheal transplant integration, we quantified the populations of host cells in the graft and native trachea following implant. Tracheal transplant, tracheal replacement, regenerative medicine, animal model. Tracheal grafts were obtained from female C57BL/6 mice and orthotopically transplanted into syngeneic male recipients. Cohorts were euthanized on day 14, day 45, and day 90 post-transplantation. Host and graft tracheas were explanted and analyzed by histology. Male host cells were quantified using fluorescence in situ hybridization, and macrophages were quantified with immunofluorescence. Evidence of host-derived cells was found in the midgraft at the earliest time point (14 days). Host-derived cells transiently increased in the graft on day 45 and were predominantly found in the submucosa. By day 90, the population of host-derived cells population declined to a similar level on day 14. Macrophage infiltra...Continue Reading
References
Jun 27, 1992·Lancet·T E StarzlM Trucco
Mar 27, 1998·Current Opinion in Hematology·R Parkman
May 23, 2002·Transplantation·Eric M GendenLloyd Mayer
Jul 19, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Isabel P NeuringerScott H Randell
Jan 15, 2003·Archives of Otolaryngology--head & Neck Surgery·Eric M GendenLloyd Mayer
Oct 15, 2003·Transplantation·Ning QuJochum Prop
Dec 7, 2007·The Journal of Clinical Investigation·Ashok N BabuMark R Nicolls
Jan 2, 2013·Frontiers in Bioscience (Elite Edition)·Alejandro Cano BribriescoDaniel Kreisel
Apr 27, 2013·Transplant Immunology·Kai FanKe Jiang
Jun 19, 2013·Transplantation Proceedings·C KonoedaT Murakawa
Jul 15, 2016·The Annals of Otology, Rhinology, and Laryngology·Tendy ChiangChristopher K Breuer
Feb 24, 2017·Annals of Thoracic and Cardiovascular Surgery : Official Journal of the Association of Thoracic and Cardiovascular Surgeons of Asia·Ahmed A AbouarabAhmed El Nori
Apr 16, 2019·Journal of Visualized Experiments : JoVE·Matthew G WietTendy Chiang
Nov 11, 2019·Acta Biomaterialia·Sayali DharmadhikariTendy Chiang