Spatial and temporal analysis of the Drosophila FMRFamide neuropeptide gene product SDNFMRFamide: evidence for a restricted expression pattern

Neuropeptides
Ruthann NicholsJ Starkman

Abstract

The expression of SDNFMRFamide, one of five different FMRFamide-containing peptides encoded by the Drosophila melanogaster FMRFamide gene, has been determined. To study expression, we generated antisera to the N-terminus of SDNFMRFamide to avoid crossreactivity with FMRFamide-containing peptides. The antisera were purified and the specificity characterized. SDNFMRFamide immunoreactive material is present in the central nervous system throughout development. Immunoreactivity is first observed in embryonic neural tissue in a cluster of cells in the subesophageal ganglion and immunoreactive fibers projecting from these cells to the brain and ventral ganglion. This pattern of expression is also observed in neural tissue dissected from larva, pupa, and adult. Double-labelling experiments indicate that cells recognized by SDNFM-antisera are also stained with FMRFamide antisera. Based on position, SDNFMRFamide immunoreactive material is expressed in a limited number of cells that contain the FMRFamide polypeptide precursor. This finding suggests that the Drosophila FMRFamide precursor undergoes differential post-translational processing.

References

Aug 12, 1977·Science·D A Price, M J Greenberg
May 1, 1990·DNA and Cell Biology·A C ChinR H Scheller
May 22, 1986·The Journal of Comparative Neurology·K WhiteP Punsal
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L E Schneider, P H Taghert
Dec 2, 1983·Science·D T Krieger
Jan 1, 1984·Annual Review of Neuroscience·Y P LohH Gainer
Nov 15, 1993·The Journal of Comparative Neurology·L E SchneiderP H Taghert
Dec 8, 1993·The Journal of Comparative Neurology·J McCormick, R Nichols
Jun 1, 1993·Neuropeptides·M F Tibbetts, R Nichols

❮ Previous
Next ❯

Citations

Dec 1, 1996·Current Opinion in Neurobiology·D R Nässel
Feb 17, 2000·Annals of the New York Academy of Sciences·R NicholsI A Lim
Jun 20, 2000·Journal of Neurogenetics·R NicholsC Rosario
Apr 16, 2002·The Journal of Comparative Neurology·Dan LuJane L Witten
Jun 11, 1999·Journal of Neurobiology·R NicholsI Lim
May 20, 1999·Microscopy Research and Technique·P H Taghert
May 7, 2002·The Journal of Comparative Neurology·Sarah WiseKathleen K Siwicki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.