Spatial and temporal expression of surfactant proteins in hyperoxia-induced neonatal rat lung injury.

BMC Pulmonary Medicine
Simone A J ter HorstGerry T M Wagenaar

Abstract

Bronchopulmonary dysplasia, a complex chronic lung disease in premature children in which oxidative stress and surfactant deficiency play a crucial role, is characterized by arrested alveolar and vascular development of the immature lung. The spatial and temporal patterns of expression of surfactant proteins are not yet fully established in newborn infants and animal models suffering from BPD. We studied the mRNA expression of surfactant proteins (SP) A, -B, -C and -D and Clara cell secretory protein (CC10) with RT-PCR and in situ hybridization and protein expression of CC10, SP-A and -D with immunohistochemistry in the lungs of a preterm rat model, in which experimental BPD was induced by prolonged oxidative stress. Gene expression of all surfactant proteins (SP-A, -B, -C and -D) was high at birth and initially declined during neonatal development, but SP-A, -B, and -D mRNA levels increased during exposure to hyperoxia compared to room-air controls. Peak levels were observed in adult lungs for SP-A, SP-C and CC10. Except for SP-A, the cellular distribution of SP-B, -C, -D and CC10, studied with in situ hybridization and/or immunohistochemistry, did not change in room air nor in hyperoxia. Exposure to normoxia was associated wi...Continue Reading

References

Jun 15, 1992·European Journal of Biochemistry·T Lacaze-MasmonteilA Kahn
Dec 11, 1991·American Journal of Respiratory Cell and Molecular Biology·S HorowitzE R Cheng
Dec 1, 1991·American Journal of Respiratory Cell and Molecular Biology·K A Veness-MeehanS Horowitz
Feb 1, 1991·American Journal of Respiratory Cell and Molecular Biology·L M NogeeJ A Whitsett
Aug 1, 1989·American Journal of Respiratory Cell and Molecular Biology·L M NogeeJ A Whitsett
Aug 1, 1989·Journal of Applied Physiology·P C EngstromS Matalon
Feb 1, 1987·Journal of Applied Physiology·S MatalonR H Notter
Nov 1, 1985·Journal of Applied Physiology·B A HolmS Matalon
May 1, 1983·Pediatric Research·R J RobertsJ R Bucher
Jun 1, 1995·American Journal of Respiratory and Critical Care Medicine·W E NovotnyB A Holm
Jan 1, 1997·Biology of the Neonate·J J Coalson
Dec 10, 1999·Pediatric Research·A J Jobe
Jun 13, 2001·American Journal of Respiratory and Critical Care Medicine·A H Jobe, E Bancalari
Jul 27, 2001·American Journal of Respiratory Cell and Molecular Biology·C W WhiteJ M Shannon
Jun 23, 2004·Journal of Applied Physiology·Simone A J ter HorstFrans J Walther
Oct 22, 2004·Pediatric Research·Jeffrey D MerrillPhilip L Ballard
Jun 2, 2005·Seminars in Fetal & Neonatal Medicine·Howard Clark, Lucy Side Clark

❮ Previous
Next ❯

Citations

Mar 10, 2012·Antioxidants & Redox Signaling·Rajesh S AlphonseBernard Thébaud
Jul 14, 2010·American Journal of Respiratory and Critical Care Medicine·Yvonne P de VisserGerry T M Wagenaar
Jun 28, 2011·Critical Care : the Official Journal of the Critical Care Forum·James DevaneyJohn G Laffey
May 16, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Cheng CaiWei Liu
May 18, 2012·Pediatrics International : Official Journal of the Japan Pediatric Society·Weihua JiXindong Xue
Jan 21, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Megan O'ReillyFoula Sozo
Oct 16, 2018·Medical Decision Making : an International Journal of the Society for Medical Decision Making·Aasthaa Bansal, Patrick J Heagerty
Aug 23, 2020·Metabolites·Abigail L PetersonHongwei Yao
Aug 4, 2021·Allergy·Martine MootzUlrich M Zissler

❮ Previous
Next ❯

Methods Mentioned

BETA
hysterectomy
electrophoresis
PCR
in vitro transcription
bronchoalveolar lavage
bronchoalveolar
ELISA
lavage
bronchoalveolar lavages
lavages

Software Mentioned

ABI Prism 7700 Sequence Detection System
Primer [UNK]
primer3

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.