Jul 13, 2006

Spatial and temporal expression of the Grainyhead-like transcription factor family during murine development

Gene Expression Patterns : GEP
Alana AudenStephen M Jane

Abstract

The Drosophila transcription factor Grainyhead (grh) is expressed in ectoderm-derived tissues where it regulates several key developmental events including cuticle formation, tracheal elongation and dorsal closure. Our laboratory has recently identified three novel mammalian homologues of the grh gene, Grainyhead-like 1, -2 and -3 (Grhl1-3) that rewrite the phylogeny of this family. Using gene targeting in mice, we have shown that Grhl3 is essential for neural tube closure, skin barrier formation and wound healing. Despite their extensive sequence homology, Grhl1 and Grhl2 are unable to compensate for loss of Grhl3 in these developmental processes. To explore this lack of redundancy, and to gain further insights into the functions of this gene family in mammalian development we have performed an extensive in situ hybridisation analysis. We demonstrate that, although all three Grhl genes are highly expressed in the developing epidermis, they display subtle differences in the timing and level of expression. Surprisingly, we also demonstrate differential expression patterns in non-ectoderm-derived tissues, including the heart, the lung, and the metanephric kidney. These findings expand our understanding of the unique role of Grhl3...Continue Reading

  • References12
  • Citations60

Mentioned in this Paper

Embryo
Entire Oral Cavity
Ethanol
Entire Atrioventricular Canal
Nasal Cavity
Myristica fragrans
Ethanol Measurement
Grainyhead-like 1 protein, Xenopus
Esophageal Tissue
Lung

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