Spatial and temporal expression of zebrafish glutathione peroxidase 4 a and b genes during early embryo development

Gene Expression Patterns : GEP
Mario A Mendieta-SerranoEnrique Salas-Vidal

Abstract

Antioxidant cellular mechanisms are essential for cell redox homeostasis during animal development and in adult life. Previous in situ hybridization analyses of antioxidant enzymes in zebrafish have indicated that they are ubiquitously expressed. However, spatial information about the protein distribution of these enzymes is not available. Zebrafish embryos are particularly suitable for this type of analysis due to their small size, transparency and fast development. The main objective of the present work was to analyze the spatial and temporal gene expression pattern of the two reported zebrafish glutathione peroxidase 4 (GPx4) genes during the first day of zebrafish embryo development. We found that the gpx4b gene shows maternal and zygotic gene expression in the embryo proper compared to gpx4a that showed zygotic gene expression in the periderm covering the yolk cell only. Following, we performed a GPx4 protein immunolocalization analysis during the first 24-h of development. The detection of this protein suggests that the antibody recognizes GPx4b in the embryo proper during the first 24 h of development and GPx4a at the periderm covering the yolk cell after 14-somite stage. Throughout early cleavages, GPx4 was located in b...Continue Reading

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Citations

Apr 19, 2018·Molecular Biology and Evolution·Gaurab K SarangiSergi Castellano
May 29, 2018·Genes & Development·Marcus ConradBrent R Stockwell
Nov 9, 2018·Antioxidants·Christine RamponSophie Vriz

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