Spatial and temporal immunocytochemical analysis of drosulfakinin (Dsk) gene products in the Drosophila melanogaster central nervous system

Cell and Tissue Research
R Nichols, I A Lim

Abstract

The spatial and temporal distribution of three peptides, DSK I, DSK II, and DSK 0, encoded by the Drosophila melanogaster drosulfakinin (Dsk) gene, have been examined in the central nervous system. DSK I and DSK II have a -RFamide C-terminus and are structurally similar to sulfakinin peptides; in contrast, DSK 0 contains -SFamide and is not structurally similar to sulfakinins. Antisera specificities were determined by the design of the antigens and confirmed by dot blot analysis and preincubation with peptides prior to their use in immunocytochemistry. The distribution of immunoreactivity suggests that all three DSK peptides are processed from the polypeptide precursor and expressed in many of the same cells. Expression was observed at all developmental stages with an increase in the level of staining and the number of immunoreactive cells as development progresses. Cells in the brain lobe, optic lobe, subesophageal ganglion, thoracic ganglia, and the eighth abdominal neuromere contain DSK-immunoreactive materials. Immunoreactive fibers project from some cells and extend into the brain and ventral ganglion with regions of extensive arborization. DSK 0 immunoreactivity provides initial evidence for the presence of a -SFamide pep...Continue Reading

Citations

Feb 17, 2000·Annals of the New York Academy of Sciences·R NicholsI A Lim
Jan 8, 2015·Frontiers in Endocrinology·Dick R Nässel, Michael J Williams
Apr 16, 2002·The Journal of Comparative Neurology·Dan LuJane L Witten
Jan 27, 2018·Journal of the American Society for Mass Spectrometry·Max DiesnerSusanne Neupert
Jun 11, 1999·Journal of Neurobiology·R NicholsI Lim
Oct 31, 2008·Cell and Tissue Research·Jan A VeenstraAzza Sellami
Sep 4, 2009·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Rami Burstein, M Jakubowski
Oct 8, 1998·The Journal of Comparative Neurology·A Malick, R Burstein
Feb 9, 2002·The Journal of Comparative Neurology·Shin-Ichi Ito
Jan 5, 2020·The Journal of Experimental Biology·Pavan AgrawalLoren L Looger
Mar 9, 2002·Clinical and Experimental Hypertension : CHE·Abraham A Palmer, Morton P Printz
Mar 16, 2004·The European Journal of Neuroscience·Katsushige SatoYoko Momose-Sato
Mar 15, 2008·Endocrinology·Tom JanssenLiliane Schoofs

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