Spatial and temporal localization of WNT signaling proteins in a mouse model of distraction osteogenesis.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Bahar KasaaiReggie C Hamdy

Abstract

While the surgical procedure of distraction osteogenesis (DO) is very successful in the treatment of orthopedic conditions, its major limitation of slow bone formation in the distracted gap has prompted numerous attempts to understand and accelerate this slow bone formation. Interestingly, WNT/FZD signaling has been identified as a critical pathway in mediating bone formation and regeneration but has not yet been studied in the context of DO. The objective of this study was to determine the spatial and temporal localization of endogenous WNT signaling proteins at various times of bone formation in a wild-type mouse model of DO. In this study, the DO protocol performed on mice consisted of three phases: latency (5 days), distraction (12 days), and consolidation (34 days). Our immunohistochemical findings of distracted bone specimens show an increased expression of WNT ligands (WNT4 and WNT10A), receptors (FZD1 and 2, LRP5 and 6), β-catenin, and pathway antagonizers (DKK1; CTBP1 and 2; sFRP1, 2, and 4) during the distraction phase, which were then down-regulated during consolidation. This is the first published report to show an activation of the WNT pathway in DO and could help identify WNT as a potential therapeutic target in a...Continue Reading

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Citations

Jun 27, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Asim M MakhdomReggie C Hamdy
Jan 30, 2015·Cellular and Molecular Neurobiology·Wenwen WangHuaijin Guan
Mar 28, 2017·Connective Tissue Research·Xuemei WangShujuan Zou
Dec 7, 2016·The Journal of Craniofacial Surgery·Chuang ZhaoEn Luo
Jun 26, 2017·Journal of Bone and Mineral Metabolism·Mohammad M AlzahraniReggie C Hamdy
Oct 31, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E'Lissa M FloresRichard P Phipps
Feb 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Santu K SinghaZia Shariat-Madar
Jun 1, 2017·The Journal of Craniofacial Surgery·Christopher M Runyan, Kyle S Gabrick

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Methods Mentioned

BETA
nuclear translocation
X-ray

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