PMID: 8589738Jan 1, 1996Paper

Spatial and temporal regulation of a lacZ reporter transgene in a binary transgenic mouse system

Transgenic Research
D P GardnerC Kappen

Abstract

The transgenic mouse system is a powerful tool for the study of gene function. However, when the analysis involves genes that are critical for the normal developmental process, the usefulness of transgenic mouse systems is limited (for review see Hanahan, 1989; Westphal and Gruss, 1989; Byrne et al., 1991). This is due to potential transgene interference with development in case of ectopic or high level expression. As a result, establishing permanent transgenic mouse lines expressing these types of genes has proven difficult. To circumvent these difficulties, a binary transgenic mouse system has been established, termed the Multiplex System (Byrne and Ruddle, 1989). This is a two-tiered gene activation system in which expression of the gene of interest occurs only in offspring carrying transgenes encoding both components: transactivator and transresponder. Transactivator lines contain the gene encoding the VP16 protein of herpes simplex virus. Transresponder lines harbour the gene of interest linked to the IE promoter which includes recognition sequences for the VP16 transactivator. Previously, the inducibility of a chloramphenicol acetyltransferase reporter gene in newborn offspring that carried both a transactivator and trans...Continue Reading

References

Jan 24, 1992·Cell·W McGinnis, R Krumlauf
Dec 11, 1992·The Journal of Experimental Zoology·S KaurS S Potter
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D M OrnitzP Leder
Aug 11, 1991·Nucleic Acids Research·P W LairdA Berns
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C J BieberichF H Ruddle
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G W Byrne, F H Ruddle
Jan 1, 1989·Annual Review of Cell Biology·H Westphal, P Gruss
Dec 8, 1989·Science·D Hanahan
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J L McKnightB Roizman
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T Gerster, R G Roeder
Mar 13, 1987·Science·M F UtsetW McGinnis
Jan 1, 1988·Genes & Development·H Le MouellicP Brûlet
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T M Kristie, B Roizman
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·P A FurthL Hennighausen
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·A C WilsonW Herr

❮ Previous
Next ❯

Citations

Jan 16, 2003·Biochimica Et Biophysica Acta·Claudia Kappen, Paul J Yaworsky
Aug 5, 1998·Biochimica Et Biophysica Acta·C H RundleC Kappen
Aug 6, 1999·Biochimica Et Biophysica Acta·D A Zacharias, C Kappen
Sep 12, 2001·Trends in Endocrinology and Metabolism : TEM·F J DeMayo, S Y Tsai
Nov 30, 2000·Nucleic Acids Research·E Fuhrmann-BenzakeinP L Herrera
Jul 15, 2009·The International Journal of Developmental Biology·Claudia Kappen, J Michael Salbaum
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y G YuehC Kappen
May 10, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yunhua Li MullerClaudia Kappen
Apr 1, 2005·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Claudia Kappen
Apr 27, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·Kimberly ChrismanDavid P Gardner
Oct 5, 2001·Nature Reviews. Genetics·M Lewandoski
Dec 5, 2002·Physiological Genomics·Ernesto BockampBernhard Zabel
Dec 22, 1999·Molecular Reproduction and Development·Y G YuehC Kappen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
G W Byrne, F H Ruddle
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Yunhua Li MullerC Kappen
© 2021 Meta ULC. All rights reserved