Spatial and temporal tracking of cardiac exosomes in mouse using a nano-luciferase-CD63 fusion protein.

Communications Biology
Weijia LuoJiang Chang

Abstract

Exosomes are secreted extracellular vesicles with lipid bilayer membranes. They are emerging as a new category of messengers that facilitate cross-talk between cells, tissues, and organs. Thus, a critical demand arises for the development of a sensitive and non-invasive tracking system for endogenous exosomes. We have generated a genetic mouse model that meets this goal. The Nano-luciferase (NanoLuc) reporter was fused with the exosome surface marker CD63 for exosome labeling. The cardiomyocyte-specific αMHC promoter followed by the loxP-STOP-loxP cassette was engineered for temporal and spatial labeling of exosomes originated from cardiomyocytes. The transgenic mouse was bred with a tamoxifen-inducible Cre mouse (Rosa26Cre-ERT2) to achieve inducible expression of CD63NanoLuc reporter. The specific labeling and tissue distribution of endogenous exosomes released from cardiomyocytes were demonstrated by luciferase assay and non-invasive bioluminescent live imaging. This endogenous exosome tracking mouse provides a useful tool for a range of research applications.

References

Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bakhos A TannousXandra O Breakefield
Aug 10, 2005·Blood Cells, Molecules & Diseases·Alain DelcayreJean-Bernard Le Pecq
Feb 17, 2009·Nature Medicine·Elmer B SantosRenier J Brentjens
Dec 8, 2009·Nature Cell Biology·Matias OstrowskiClotilde Thery
Jun 24, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dongmei SunHuang-Ge Zhang
Aug 13, 2011·Circulation Research·Susmita SahooDouglas W Losordo
May 15, 2012·Circulation Research·Alexander R MackieDouglas W Losordo
Aug 28, 2012·Advanced Drug Delivery Reviews·Atsushi SuetsuguRobert M Hoffman
Dec 12, 2012·Molecular & Cellular Proteomics : MCP·Bow J TauroRichard J Simpson
Sep 7, 2013·Journal of Extracellular Vesicles·Yusuke YoshiokaTakahiro Ochiya
Feb 28, 2014·International Journal of Molecular Medicine·Cristina GrangeGiovanni Camussi
Apr 20, 2014·The Journal of Clinical Investigation·Claudia BangThomas Thum
May 16, 2014·Journal of Molecular and Cellular Cardiology·Xiaohong WangGuo-Chang Fan
Oct 4, 2014·Frontiers in Immunology·Zoraida Andreu, María Yáñez-Mó
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Marina ColomboClotilde Théry
Feb 12, 2015·Journal of Extracellular Vesicles·Takafumi ImaiYoshinobu Takakura
Apr 18, 2015·Journal of the American College of Cardiology·Jose M VicencioSean M Davidson
Apr 23, 2015·Journal of Extracellular Vesicles·Oscar P B WiklanderSamir El Andaloussi
Dec 15, 2015·Nature Protocols·Anoek ZomerJacco van Rheenen
Dec 17, 2015·Annual Review of Physiology·Ahmed Ibrahim, Eduardo Marbán
Apr 2, 2016·The Journal of Clinical Investigation·Raghu Kalluri
Apr 17, 2016·Stem Cell Research & Therapy·Hongyoon Choi, Dong Soo Lee
Jan 8, 2017·Journal of Cellular Physiology·Fahimeh ShahabipourAmirhossein Sahebkar
Feb 1, 2017·Cell Adhesion & Migration·Vincent HyenneJacky G Goetz
Feb 17, 2017·Pharmacology & Therapeutics·Lucio Barile, Giuseppe Vassalli
Mar 2, 2017·BioMed Research International·Prakash GangadaranByeong-Cheol Ahn
Mar 17, 2017·Circulation Research·Prabhu MathiyalaganSusmita Sahoo
Mar 21, 2017·The Journal of Clinical Investigation·Prashanth VallabhajosyulaAli Naji
Jul 25, 2017·Cellular and Molecular Life Sciences : CMLS·Nina Pettersen Hessvik, Alicia Llorente
Sep 2, 2017·Cardiovascular Research·Teresa M Ribeiro-RodriguesHenrique Girão
Dec 14, 2017·Frontiers in Molecular Biosciences·Paula Carpintero-FernándezAna O'Loghlen
Jan 13, 2018·Journal of the American College of Cardiology·Eduardo Marbán
Mar 30, 2018·Circulation Research·Xavier LoyerChantal M Boulanger
May 29, 2018·Molecular Therapy : the Journal of the American Society of Gene Therapy·Marta Adamiak, Susmita Sahoo

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Citations

Nov 4, 2020·Trends in Molecular Medicine·Tania Martins-MarquesHenrique Girao
Apr 4, 2021·International Journal of Molecular Sciences·Nienke M StegeHerman H W Silljé
Apr 8, 2021·Cell Death & Disease·Yaodan ZhangQiang Zhang
Jun 1, 2021·Advanced Drug Delivery Reviews·Alexandre AndrouinGuillaume van Niel
Jun 5, 2021·Frontiers in Pharmacology·Lalitha Sarad Yamini NanduriChandan Guha
May 21, 2021·Advanced Drug Delivery Reviews·Pol Escudé Martinez de CastillaJiong-Wei Wang
Apr 17, 2021·Proteomics·Bethany ClaridgeDavid W Greening
Oct 28, 2021·Advanced Healthcare Materials·Bryan Z WangGordana Vunjak-Novakovic

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
fluorescence imaging
transgenic
genotyping
PCR
transmission electron microscopy
xenograft

Software Mentioned

GraphPad Prism
NTA3

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