Spatial and temporal variability of ribotyping results at a small watershed in South Carolina

Water Research
R Heath KelseyGeoffrey I Scott

Abstract

The utility of library-based ribotyping methods for a very small study area was evaluated through comparison of local results to libraries with differing spatial and temporal scales. Ribotyping of Escherichia coli isolates was used to evaluate sources of fecal pollution at a coastal golf course in Beaufort County, South Carolina. Thirty-five E. coli isolates were obtained from water samples from a detention pond for testing against several local and regional libraries of known-source isolate patterns. A library of 92 E. coli ribotype patterns was created from wildlife feces obtained on the site. Additional libraries were available for comparison, including a library from Morgan Island, a small, geographically isolated area (including a monkey colony), and a library from ongoing statewide assessments. Seventeen (49%) of the unknown E. coli isolates matched isolates from raccoon and deer scat from the local library. Two isolates (6%) were matched with monkey sources from Morgan Island, and 13 (37%) were matched to raccoon, deer, and cows from the statewide assessment. Evaluation of repeated ribotyping analyses at the study area revealed evidence of temporal variability of potential sources in the local library. Only one of the is...Continue Reading

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Citations

Oct 4, 2011·Applied and Environmental Microbiology·Melanie WickiAndreas Baumgartner
Feb 26, 2013·Environmental Monitoring and Assessment·L F WebsterM H Fulton
Oct 22, 2013·The Science of the Total Environment·Susana AraújoAntónio Correia

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