Spatial composition of prostate cancer spheroids in mixed and static cultures

Tissue Engineering
Hong SongKim C O'Connor

Abstract

Aggregation of neoplastic cells produces multicellular spheroids resembling micrometastases. The objective of this study was to investigate the effects of mixing culture medium on the spatial composition of spheroids prepared from well (LNCaP) and poorly (DU 145) differentiated human prostate cancer cells. Spheroids were cultured in a mixed suspension within a high-aspect rotating wall vessel and static liquid-overlay plate. Results from this study demonstrate that mixed cultures consistently manifested differences in morphology and composition between DU 145 and LNCaP spheroids. For example, 40 +/- 12% of DU 145 cells were Ki-67 positive 100 microm from the surface within mixed spheroids versus 0% for LNCaP cells; there was no significant difference in this spatial profile for static cultures. The results suggest that poorly differentiated spheroids may be more likely to experience a change in composition from mixing culture medium than well-differentiated spheroids, due to low tissue density. Immunostaining for P-glycoprotein is representative of this trend; average staining intensity increased 50% for DU 145 spheroids on mixing but was unchanged for LNCaP spheroids. The effects of mixing on spheroid composition were attribut...Continue Reading

References

Jul 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R DahiyaP Narayan
Oct 15, 1987·International Journal of Cancer. Journal International Du Cancer·M KitaharaT Sasaki
May 1, 1997·In Vitro Cellular & Developmental Biology. Animal·P I LelkesT J Goodwin
Mar 20, 1998·International Journal of Cancer. Journal International Du Cancer·M WartenbergH Sauer
Sep 19, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·R K Jain
Sep 24, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J P van BrusselG H Mickisch
Aug 17, 2000·Journal of the National Cancer Institute·P BorstJ Wijnholds
Aug 19, 2000·In Vitro Cellular & Developmental Biology. Animal·J M JessupT J Goodwin
Feb 17, 2001·Biotechnology and Bioengineering·K J GoochG Vunjak-Novakovic
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M WartenbergH Sauer
Jan 30, 2002·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael Thun
Jul 19, 2002·Journal of Biomedical Materials Research·Vassilios I SikavitsasAntonios G Mikos
Aug 1, 2002·Oncogene·Qimin ZhanRajasekaran Baskaran
Oct 2, 2002·Biotechnology and Bioengineering·Richard M EnmonDaniel K Schwartz
Feb 13, 2003·The Journal of Pathology·Michael D SlaterJulian A Barden
Jun 5, 1996·Biotechnology and Bioengineering·S ClejanA C Primavera

❮ Previous
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Citations

Mar 12, 2010·Tissue Engineering. Part a·Jun-Ichi SasakiHirofumi Yatani
Feb 10, 2009·Tissue Engineering. Part C, Methods·Jed JohnsonJohn J Lannutti
Jul 16, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Can SarisozenVladimir P Torchilin
Dec 7, 2007·International Journal of Radiation Biology·Juergen FriedrichLeoni A Kunz-Schughart
Sep 2, 2014·Advanced Drug Delivery Reviews·Maureen E Lynch, Claudia Fischbach
May 23, 2015·Therapeutic Delivery·Niravkumar R PatelVladimir P Torchilin
Jun 16, 2009·Biomaterials·Jungwoo LeeNicholas A Kotov
Mar 16, 2017·Journal of Cardiovascular Translational Research·Isotta ChimentiElisa Messina
Feb 12, 2017·Lab on a Chip·Yuta KurashinaJames Friend
Jun 21, 2008·Biotechnology Journal·Ruei-Zeng LinHwan-You Chang
Nov 21, 2014·Advanced Functional Materials·Ehsan AtefiHossein Tavana
Oct 29, 2018·Journal of Biomedical Materials Research. Part a·Xiaolin CuiHu Zhang
May 11, 2019·PloS One·Michael E BregenzerGeeta Mehta
May 13, 2011·Biofabrication·A Nagy MeheszV Mironov
Apr 15, 2014·Biofabrication·Chengyang WangWei Sun
Jul 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yoshitaka SuematsuToshinori Fujie

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