Spatial distribution of ochratoxin A in vineyard and sampling design to assess must contamination

Journal of Food Protection
Paola BattilaniAmedeo Pietri

Abstract

The aim of this work was to study the spatial variability of ochratoxin A (OTA) in vineyards and to define a reliable sampling protocol for bunches in order to assess OTA content before harvesting. In 2002, two vineyards with 'Negroamaro' and 'Sangiovese' grape varieties were chosen in Southern Italy. The same sampling design was applied to both vineyards. Ten plants were collected from the X-shaped path of the whole vineyard (plants 1 through 10) and 10 plants from the two central cross-perpendicular lines (plants 11 through 20). Bunches harvested from plants 1 through 10 were numbered progressively, weighed, and crushed separately, and bunches obtained from plants 11 through 20 were crushed plant by plant. Juices obtained were analyzed for OTA content by high-performance liquid chromatography. Then, a simulated approach for sampling was applied, following randomized and systematic designs. High and random variability was observed in OTA content both among bunches and among plants in the two vineyards, independent of contamination level. Simulated sampling design markedly influenced the assessment of must contamination. The best results were obtained when sampling involved one bunch per plant, in a predefined position, from at...Continue Reading

References

Feb 1, 1966·Journal of the American Oil Chemists' Society·A F CuculluL A Goldblatt
Aug 1, 1996·Food Additives and Contaminants·B Zimmerli, R Dick
Oct 29, 2000·Methods in Molecular Biology·T B Whitaker
Apr 17, 2003·Journal of Food Protection·Paola BattilaniZofia Kozakiewicz

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