PMID: 11933011Apr 5, 2002Paper

Spatial distribution patterns of interphase centromeres during retinoic acid-induced differentiation of promyelocytic leukemia cells

Cytometry
M BeilTheano Irinopoulou

Abstract

The pericentromeric heterochromatin is an important element for the regulation of gene silencing. Its spatial distribution during interphase appears to be cell-type specific. This study analyzes three-dimensional (3D) centromere distribution patterns during cellular differentiation along the neutrophil pathway. Differentiation of the promyelocytic leukemia cell line NB4 was induced by retinoic acid. Centromeres in interphase nuclei were visualized by immunofluorescence staining of centromere-associated proteins with CREST serum. 3D images of nuclei were obtained by confocal microscopy. Automated methods for the segmentation of point-like objects in 3D images were implemented to detect the position of centromeres. Features of centromere localization patterns were determined by constructing the minimal spanning tree of the centromere distribution. In differentiated NB4 cells, the number of centromere conglomerates (chromocenters) was decreased and the distance between chromocenters was increased as compared with untreated controls. The nuclear volume did not differ between the two groups. The measured rearrangement of centromeres indicates a progressive clustering of heterochromatin and a global remodeling of interphase chromosom...Continue Reading

References

Feb 1, 1991·Experimental Cell Research·T Haaf, M Schmid
Apr 7, 1987·Journal of Theoretical Biology·C DussertF Marty
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Manuelidis
Jun 1, 1993·Current Opinion in Cell Biology·D L Spector
May 1, 1993·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·F ParazzaY Usson
Mar 15, 1996·Experimental Cell Research·N Chaly, S B Munro
Jan 1, 1996·Critical Reviews in Eukaryotic Gene Expression·L de JongR van Driel
Nov 1, 1996·The Journal of Cell Biology·R D ShelbyK F Sullivan
Aug 7, 1997·Journal of Theoretical Biology·F Wallet, C Dussert
Oct 9, 1997·The New England Journal of Medicine·M S TallmanP H Wiernik
Mar 24, 1998·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·S DietzelT Cremer
May 9, 1998·Science·A I Lamond, W C Earnshaw
Sep 1, 1986·Physical Review. B, Condensed Matter·C DussertA Llebaria
May 14, 1999·Current Opinion in Genetics & Development·K W DobieG H Karpen
May 14, 1999·Current Opinion in Genetics & Development·M Cockell, S M Gasser
Jul 15, 1999·Cytometry·M KozubekA Jergová
Oct 6, 1999·The Journal of Cell Biology·P J VerschureR van Driel
Feb 5, 2000·Molecular Reproduction and Development·M HazzouriB Sèle
Apr 8, 2000·Current Opinion in Genetics & Development·J C Eissenberg, S C Elgin
Oct 14, 2000·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M SkalníkováM Kozubek
Jan 1, 1998·Journal of Cellular Biochemistry·Ronald Berezney, Xiangyun Wei

❮ Previous
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Citations

Apr 21, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Valeria MericoSilvia Garagna
Sep 6, 2003·Gene·Marta AlvarezJoseph P Bidwell
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Franziska LautenschlägerJochen Guck
Sep 22, 2007·American Journal of Respiratory Cell and Molecular Biology·Anne DruilheMarina Pretolani
Aug 7, 2007·PLoS Computational Biology·Carol ShielsPaul S Freemont
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·David W KnowlesSophie A Lelièvre
Jun 9, 2005·The Journal of Cell Biology·Alessandro BreroM Cristina Cardoso
Nov 17, 2009·Comptes rendus biologies·Valérie GaudinPascale Debey
Jul 25, 2009·Journal of Cellular Physiology·Narasimharao V MarellaRonald Berezney
May 22, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Arkadiusz GertychJian Tajbakhsh
May 18, 2006·Biology of the Cell·Eva Bártová, Stanislav Kozubek
Apr 22, 2008·Journal of Cellular Biochemistry·Amanda Gonçalves Dos Santos SilvaSabine Mai
Sep 1, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Antonina MaslovaAlla Krasikova
Sep 27, 2005·Experimental Cell Research·Rémi TerranovaAmanda G Fisher
May 28, 2014·BioMed Research International·Martina BelliMaurizio Zuccotti
Jun 3, 2020·Genes·Salvatore FiorinielloFloriana Della Ragione
Jan 8, 2020·International Journal of Molecular Sciences·Jekaterina Erenpreisa, Alessandro Giuliani
Jan 17, 2021·Biophysical Journal·Jekabs KrigertsJekaterina Erenpreisa
Apr 5, 2007·Journal of Cell Science·Gurushankar ChandramoulySophie A Lelièvre
Aug 28, 2004·Journal of Cell Science·Katharine L Arney, Amanda G Fisher
Apr 18, 2015·Genome Research·Patrick J WijchersWouter de Laat

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