Spatial distributions of sulfur-rich proteins in cornifying epithelia

Journal of Structural Biology
R D LeapmanA C Steven

Abstract

We have used energy-filtered electron microscopy and electron energy loss spectroscopy (EELS) to characterize the distributions of sulfur-rich proteins in granular layer cells of squamous stratifying epithelia and their redistribution in the cornified layer, with particular attention to assembly of the cornified cell envelope (CE). Our measurements provide quantitative information that complements highly specific but qualitative data from immunocytochemistry. Spatial distributions of sulfur, phosphorus, and nitrogen were mapped in unstained thin sections of mouse epidermis and forestomach, using a postcolumn energy filter. Nitrogen images were indicative of total protein while phosphorus images provided a control to validate the algorithms used to calculate the elemental maps. Sulfur was found at high levels in round L-granules in the granulocyte cytoplasm and in the cornified CE, correlating with the presence of the protein, loricrin ( approximately 7% Cys/Met residues). EELS confirmed these observations quantitatively: either L-granules consist exclusively of loricrin or any additional components must have an equally high net sulfur content. These data also confirm the large fraction (approximately 75%) of loricrin in the CE,...Continue Reading

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Citations

Mar 2, 2013·Journal of Biological Physics·Pernilla NevstenReine Wallenberg
Oct 11, 2012·MRS Bulletin·M A Aronova, R D Leapman
Feb 20, 2002·The Journal of Investigative Dermatology·Michal JarnikAlasdair C Steven
Aug 13, 2010·Journal of Microscopy·M A AronovaR D Leapman
Feb 13, 2007·Biochimica Et Biophysica Acta·O LópezA de la Maza
Jun 9, 2016·Ultrastructural Pathology·Luca RoncatiAntonio Maiorana
Jun 2, 2004·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Anthony Z FaraneshElliot R McVeigh
Jul 8, 2003·Microscopy Research and Technique·Gertrud GopingRichard Leapman
Sep 28, 2002·Journal of Morphology·Lorenzo Alibardi
Dec 15, 2005·Zoology : Analysis of Complex Systems, ZACS·Lorenzo Alibardi
Sep 19, 2006·Ultramicroscopy·M A AronovaR D Leapman

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