Spatial fluorescence cross-correlation spectroscopy

Applied Optics
Rodolphe JaffiolJacques Derouard

Abstract

We present an alternative method for diffusion measurements of fluorescent species in solution by use of confocal microscopy and fluorescence correlation spectroscopy techniques. It consists of making a time and spatial dual correlation in which one detects the fluorescence signals from two nearby separate confocal volumes and cross correlates them. To improve the spatial discrimination between the two confocal volumes we propose filtering of fluorescence photocounts by rejecting the fluorescence background, which corresponds to particles located far from the center of the detection volumes.

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Citations

Jun 8, 2007·The Review of Scientific Instruments·Xiaotao PanThorsten Wohland
Dec 18, 2013·Annual Review of Physical Chemistry·Nirmalya Bag, Thorsten Wohland
Jan 12, 2011·Annual Review of Physical Chemistry·Hai-Tao He, Didier Marguet
Jul 2, 2009·Journal of Biomedical Optics·Céline BoutinRodolphe Jaffiol
May 28, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Sarah M SterlingDavid J Neivandt
Sep 18, 2007·Cell Biochemistry and Biophysics·Ling Chin Hwang, Thorsten Wohland
Oct 16, 2007·Biophysical Journal·Zdenek Petrásek, Petra Schwille
Feb 3, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Thomas DertingerJörg Enderlein
Apr 4, 2009·Journal of Biophotonics·Yoann BlancquaertAntoine Delon
Mar 26, 2013·Analytical Chemistry·Pascale Winckler, Rodolphe Jaffiol

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