Spatial Heterogeneity and Co-occurrence of Mucosal and Luminal Microbiome across Swine Intestinal Tract

Frontiers in Microbiology
Li ZhangHongfu Zhang

Abstract

Pigs are one of the most important economic livestock. Gut microbiota is not only critical to the health but also the production efficiency of pigs. Manipulating gut microbiota relies on the full view of gut microbiome and the understanding of drive forces shaping microbial communities. 16s rDNA sequencing was used to profile microbiota along the longitudinal and radical axes to obtain the topographical map of microbiome in different intestinal compartments in young pigs. Alpha and beta-diversities revealed distinct differences in microbial compositions between the distal ileum and cecum and colon, as well as between the lumen and mucosa.FirmicutesandProteobacteriadominated in the ileum, constituting 95 and 80% of the luminal and mucosa-attached microbiome. Transitioning from the small intestine to the large intestine, luminalBacteroidetesincreased from 1.69 to 45.98% in the cecum and 40.09% in the colon, while mucosalBacteroidetesraised from 9 to 35.36% and 27.96%. Concurrently, luminalFirmicutesandProteobacteriaand mucosal-attachedProteobacteriaremarkably decreased. By co-occurrence network analyses,Prevotellaceae, Ruminococcaceae, LachnospiraceaeandVeillonellaceaewere recognized as the central nodes of luminal microbial netw...Continue Reading

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Citations

Jul 12, 2019·FEMS Microbiology Letters·Katarzyna Śliżewska, Agnieszka Chlebicz
Oct 2, 2019·Microbial Biotechnology·Shaoming FangQianFu Gan
Dec 7, 2019·FEMS Microbiology Letters·Katarzyna Śliżewska, Agnieszka Chlebicz
Sep 3, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kent A WillisNamasivayam Ambalavanan
Jan 9, 2019·Frontiers in Veterinary Science·Christine UnterwegerIsabel Hennig-Pauka
Jun 13, 2020·Animals : an Open Access Journal From MDPI·Xizhong DuYingping Xiao
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Jan 20, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Shen-Tong WangJia-Le Li
May 18, 2021·Animal Nutrition·Lijuan FanWenkai Ren
Jun 14, 2021·Food Microbiology·M MarmionA G M Scannell
Jul 15, 2021·Animal Nutrition·Jie PengYanhua Huang

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Datasets Mentioned

BETA
PRJNA402089

Methods Mentioned

BETA
PCR
PBA

Software Mentioned

R
phyloseq
ANOSIM
SparCC
SPSS
SIMCA
SparCC ( Sparse Correlations for Compositional
Canoco
UCHIME
Qiime

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