Spatial mapping of phosphorus influx in bean root systems using digital autoradiography

Journal of Experimental Botany
Gerardo RubioJonathan P Lynch

Abstract

A novel technique was developed to spatially map the phosphorus net influx capacity in intact root systems. The method is based on digital autoradiography and permits the quantification of phosphorus influx at high spatial resolution (2 mm). Roots of 18-d-old common bean plants were exposed to (32)P-labelled orthophosphate, quickly frozen, excised, lyophilized, scanned, and exposed to a storage phosphor screen. Plots of (32)P content versus root length (distance from the root tip or from the base of the root) were obtained for three different root classes: basal, basal laterals, and taproot laterals. Radioactivity detected by filmless autoradiography correlated well (r(2)=0.99) with measurements made by scintillation counting. Basal roots absorbed 2.5 times and 1.9 times more phosphorus than the taproot lateral and basal lateral root classes, respectively, in the first 20 mm from the root apex. External phosphorus markedly affected influx: roots averaged 5, 16, and 34 pmol P min(-1) in the apical 20 mm when exposed to 1, 5, and 10 microM P solutions, respectively. The spatial pattern of phosphorus influx along the root axes of the different root classes was rather homogeneous when measured on a root surface area basis. Phosphor...Continue Reading

Citations

Mar 13, 2012·Plant Science : an International Journal of Experimental Plant Biology·Eric E Hernández-DomíguezRogelio Rodríguez-Sotres
Mar 25, 2018·Physiologia Plantarum·Clémentine PernotAnnie DesRochers
Apr 2, 2009·Rapid Communications in Mass Spectrometry : RCM·David M G AndersonMalcolm R Clench
Oct 30, 2020·Frontiers in Plant Science·Raphael TizianiStefano Cesco
Dec 31, 2020·Plants·Rosa VescioAgostino Sorgonà
Dec 25, 2015·Plant Physiology·Bianka Steffens, Amanda Rasmussen

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