Spatial Micropatterning of Growth Factors in 3D Hydrogels for Location-Specific Regulation of Cellular Behaviors

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Oju JeonEben Alsberg

Abstract

Growth factors are potent stimuli for regulating cell function in tissue engineering strategies, but spatially patterning their presentation in 3D in a facile manner using a single material is challenging. Micropatterning is an attractive tool to modulate the cellular microenvironment with various biochemical and physical cues and study their effects on stem cell behaviors. Implementing heparin's ability to immobilize growth factors, dual-crosslinkable alginate hydrogels are micropatterned in 3D with photocrosslinkable heparin substrates with various geometries and micropattern sizes, and their capability to establish 3D micropatterns of growth factors within the hydrogels is confirmed. This 3D micropatterning method could be applied to various heparin binding growth factors, such as fibroblast growth factor-2, vascular endothelial growth factor, transforming growth factor-betas and bone morphogenetic proteins while retaining the hydrogel's natural degradability and cytocompability. Stem cells encapsulated within these micropatterned hydrogels have exhibited spatially localized growth and differentiation responses corresponding to various growth factor patterns, demonstrating the versatility of the approach in controlling stem ...Continue Reading

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Citations

Jun 7, 2019·Biofabrication·Amir K MiriAli Khademhosseini
Aug 1, 2020·Advanced Healthcare Materials·Kevin T Campbell, Eduardo A Silva
Apr 11, 2020·Tissue Engineering. Part B, Reviews·Jianrui Ma, Chengyang Huang
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Tingting YuBing Han
Nov 22, 2019·Acta Biomaterialia·Hui X TanPeter J Crack
Sep 1, 2019·Nature Reviews. Materials·Michael J KratochvilSarah C Heilshorn
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Chrysanthi-Maria MoysidouRóisín Meabh Owens
Aug 1, 2020·Acta Biomaterialia·Aline LueckgenAmaia Cipitria
May 13, 2021·Stem Cell Reports·Ananya GuptaKatharina F Sonnen
Jun 5, 2021·Advanced Drug Delivery Reviews·Leon Jia Wei TangEliza Li Shan Fong
Aug 27, 2021·Bioengineering·Emilie GontranPascale Dupuis-Williams
Sep 2, 2021·Bioactive Materials·Aixiang DingEben Alsberg

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