Abstract
Growth factors are potent stimuli for regulating cell function in tissue engineering strategies, but spatially patterning their presentation in 3D in a facile manner using a single material is challenging. Micropatterning is an attractive tool to modulate the cellular microenvironment with various biochemical and physical cues and study their effects on stem cell behaviors. Implementing heparin's ability to immobilize growth factors, dual-crosslinkable alginate hydrogels are micropatterned in 3D with photocrosslinkable heparin substrates with various geometries and micropattern sizes, and their capability to establish 3D micropatterns of growth factors within the hydrogels is confirmed. This 3D micropatterning method could be applied to various heparin binding growth factors, such as fibroblast growth factor-2, vascular endothelial growth factor, transforming growth factor-betas and bone morphogenetic proteins while retaining the hydrogel's natural degradability and cytocompability. Stem cells encapsulated within these micropatterned hydrogels have exhibited spatially localized growth and differentiation responses corresponding to various growth factor patterns, demonstrating the versatility of the approach in controlling stem ...Continue Reading
References
May 1, 1992·Experimental Cell Research·C TacchettiR Cancedda
Jul 20, 2000·The Journal of Biological Chemistry·W H YuJ F Woessner
Jun 28, 2002·Nature·Hans-Peter GerberNapoleone Ferrara
Jan 8, 2005·Nature Biotechnology·M P Lutolf, J A Hubbell
Jun 15, 2006·Tissue Engineering·Robert M Nerem
Apr 25, 2008·Biomaterials·Soo-Hong LeeJennifer L West
Oct 18, 2008·Angewandte Chemie·Andrea LöberMichael R Buchmeiser
Dec 17, 2008·Current Stem Cell Research & Therapy·Mirjam FröhlichGordana Vunjak-Novakovic
Dec 8, 2009·Science·Richard O Hynes
Jan 5, 2010·Soft Matter·Alvaro MataSamuel I Stupp
Apr 27, 2010·Biomaterials·Jason W NicholAli Khademhosseini
Aug 3, 2010·Biomaterials·Sudhir Khetan, Jason A Burdick
Mar 26, 2011·Stem Cells International·Jeffery J AulettaLuis A Solchaga
Jul 13, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oju JeonEben Alsberg
Aug 30, 2011·Nature Materials·Ryan G WylieMolly S Shoichet
Nov 22, 2011·Cancer Metastasis Reviews·Samar Masoumi MoghaddamMohammad H Pourgholami
Dec 14, 2011·Angewandte Chemie·Cole A DeForest, Kristi S Anseth
Jan 26, 2012·Biotechnology Progress·Hwan Hee OhGuoping Chen
Feb 24, 2015·Nature Materials·Cole A DeForest, David A Tirrell
Jul 27, 2015·Developmental Biology·James Jabalee, Tamara A Franz-Odendaal
Sep 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Matthew B ApplegateFiorenzo G Omenetto
Nov 10, 2015·Advanced Materials·Jiyun KimKandice Tanner
Apr 19, 2016·ACS Applied Materials & Interfaces·Yuhang HuJoanna Aizenberg
Citations
Jun 7, 2019·Biofabrication·Amir K MiriAli Khademhosseini
Aug 1, 2020·Advanced Healthcare Materials·Kevin T Campbell, Eduardo A Silva
Dec 18, 2019·Biomaterials Research·Cen ChenIn-Seop Lee
Apr 11, 2020·Tissue Engineering. Part B, Reviews·Jianrui Ma, Chengyang Huang
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Tingting YuBing Han
Jun 4, 2020·Biomaterials Science·Weilue HeRupak Rajachar
Nov 22, 2019·Acta Biomaterialia·Hui X TanPeter J Crack
Sep 1, 2019·Nature Reviews. Materials·Michael J KratochvilSarah C Heilshorn
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Chrysanthi-Maria MoysidouRóisín Meabh Owens
Aug 1, 2020·Acta Biomaterialia·Aline LueckgenAmaia Cipitria
May 13, 2021·Stem Cell Reports·Ananya GuptaKatharina F Sonnen
Jun 5, 2021·Advanced Drug Delivery Reviews·Leon Jia Wei TangEliza Li Shan Fong
Dec 5, 2019·ACS Applied Materials & Interfaces·Dan WeiHongsong Fan
Aug 15, 2021·Journal of Colloid and Interface Science·Lei ChenLinge Wang
Aug 27, 2021·Bioengineering·Emilie GontranPascale Dupuis-Williams
Sep 2, 2021·Bioactive Materials·Aixiang DingEben Alsberg