PMID: 701364Sep 1, 1978Paper

Spatial orientation of glycoproteins in membranes of rat liver rough microsomes. II. Transmembrane disposition and characterization of glycoproteins

The Journal of Cell Biology
E Rodriguez BoulanG Kreibich

Abstract

Rat liver microsomal glycoproteins were purified by affinity chromatography on concanavalin A Sepharose columns from membrane and content fractions, separated from rough microsomes (RM) treated with low concentrations of deoxycholate (DOC). All periodic acid-Schiff (PAS)-positive glycoproteins of RM showed affinity for concanavalin A Sepharose; even after sodium dodecyl sulfate (SDS) acrylamide gel electrophoresis, most of the microsomal glycoproteins bound [125I]concanavalin A added to the gels, as detected by autoradiography. Two distinct sets of glycoproteins are present in the membrane and content fractions derived from RM. SDS acrylamide gel electrophoresis showed that RM membranes contain 15--20 glycoproteins (15--22% of the total microsomal protein) which range in apparent mol wt from 23,000 to 240,000 daltons. A smaller set of glycoproteins (five to seven polypeptides), with apparent mol wt between 60,000 and 200,000 daltons, was present in the microsomal content fraction. The disposition of the membrane glycoproteins with respect to the membrane plane was determined by selective iodination with the lactoperoxidase (LPO) technique. Intact RM were labeled on their outer face with 131I and, after opening of the vesicles w...Continue Reading

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Citations

Feb 1, 1989·Experimental and Molecular Pathology·A Peralta Soler, A Aoki
Mar 1, 1982·Journal of Ultrastructure Research·M Dauwalder, W G Whaley
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S Bar-NunD D Sabatini
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L LiscumM S Brown
Nov 1, 1985·The Journal of Cell Biology·R BrandsJ E Rothman
Oct 1, 1980·The Journal of Cell Biology·F Marty, D Branton
Jan 1, 1982·The Journal of Cell Biology·D D SabatiniM Adesnik
Sep 1, 1984·The Journal of Cell Biology·M G RosenfeldG Kreibich
Dec 1, 1983·European Journal of Biochemistry·M Robbi, H Beaufay
Oct 11, 2005·Annual Review of Cell and Developmental Biology·David D Sabatini
Jan 1, 1989·Drug Metabolism Reviews·B Kemper, E Szczesna-Skorupa
Jul 1, 1982·Journal of Neurochemistry·M Butler, P Morell
Jan 1, 1980·Annals of the New York Academy of Sciences·G KreibichD D Sabatini
Feb 1, 1982·Journal of Dairy Science·J C Mercier, P Gaye
Dec 24, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A M Tartakoff
Jan 20, 1987·Biochimica Et Biophysica Acta·T Brody, J W Suttie
Dec 20, 1979·Biochimica Et Biophysica Acta·R Lotan, G L Nicolson

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