Spatial pattern analysis of nuclear migration in remodelled muscles during Drosophila metamorphosis

BMC Bioinformatics
Yadav KuleeshaMartin Wasser

Abstract

Many human muscle wasting diseases are associated with abnormal nuclear localization. During metamorphosis in Drosophila melanogaster, multi-nucleated larval dorsal abdominal muscles either undergo cell death or are remodeled to temporary adult muscles. Muscle remodeling is associated with anti-polar nuclear migration and atrophy during early pupation followed by polar migration and muscle growth during late pupation. Muscle remodeling is a useful model to study genes involved in myonuclear migration. Previously, we showed that loss of Cathepsin-L inhibited anti-polar movements, while knockdown of autophagy-related genes affected nuclear positioning along the medial axis in late metamorphosis. To compare the phenotypic effects of gene perturbations on nuclear migration more objectively, we developed new descriptors of myonuclear distribution. To obtain nuclear pattern features, we designed an algorithm to detect and track nuclear regions inside live muscles. Nuclear tracks were used to distinguish between fast moving nuclei associated with fragments of dead muscles (sarcolytes) and slow-moving nuclei inside remodelled muscles. Nuclear spatial pattern features, such as longitudinal (lonNS) and lateral nuclear spread (latNS), all...Continue Reading

References

Sep 21, 2017·Journal of Biomedical Optics·Wei-Ming ChiewHock Soon Seah

Citations

Dec 1, 1989·Cell·Z W Hall, E Ralston
Sep 17, 1999·Muscle & Nerve·D L AllenV R Edgerton
Apr 28, 2001·Biological Reviews of the Cambridge Philosophical Society·T R Gregory
Jun 19, 2003·The Journal of Physiology·Jo C BruusgaardKristian Gundersen
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zhirong BaoRobert H Waterston
Apr 11, 2006·IEEE Transactions on Bio-medical Engineering·Xiaowei ChenStephen T C Wong
Apr 29, 2008·The Journal of Physiology·Kristian Gundersen, Jo C Bruusgaard
Sep 1, 2009·American Journal of Human Genetics·Lucie GueneauGisèle Bonne
Nov 4, 2009·IEEE Transactions on Bio-medical Engineering·Yousef Al-KofahiBadrinath Roysam
Mar 12, 2010·Nature·Frank SchnorrerBarry J Dickson
May 29, 2010·Annual Review of Cell and Developmental Biology·Daniel A Starr, Heidi N Fridolfsson
May 31, 2012·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Philippe ThévenazMichael Unser
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 29, 2012·The Journal of Cell Biology·Hadas Elhanany-TamirTalila Volk
Dec 19, 2014·BMC Bioinformatics·Yadav KuleeshaMartin Wasser
Dec 19, 2014·PLoS Genetics·Victoria K SchulmanMary K Baylies
Jan 8, 2015·Frontiers in Aging Neuroscience·Heinz Jungbluth, Mathias Gautel
Mar 22, 2016·Royal Society Open Science·Yadav KuleeshaMartin Wasser

Related Concepts

Establishment and Maintenance of Localization
ATG5
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In Vivo
Gene Knockdown Techniques
Genes
Nuclear Migration
Cosuppression
Drosophila melanogaster Proteins
Time-Lapse Imaging

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