Spatial Patterns and Age-Related Changes of the Collagen Crimp in the Human Cornea and Sclera

Investigative Ophthalmology & Visual Science
Alexandra GogolaIan A Sigal

Abstract

Collagen is the main load-bearing component of the eye, and collagen crimp is a critical determinant of tissue mechanical behavior. We test the hypothesis that collagen crimp morphology varies over the human cornea and sclera and with age. We analyzed 42 axial whole-globe sections from 20 normal eyes of 20 human donors, ranging in age from 0.08 (1 month) to 97 years. The sections were imaged using polarized light microscopy to obtain μm-scale fiber bundle/lamellae orientation from two corneal and six scleral regions. Crimp morphology was quantified through waviness, tortuosity, and amplitude. Whole-globe median waviness, tortuosity, and amplitude were 0.127 radians, 1.002, and 0.273 μm, respectively. These parameters, however, were not uniform over the globe, instead exhibiting distinct, consistent patterns. All crimp parameters decreased significantly with age, with significantly different age-related decreases between regions. The crimp morphology of the limbus changed the most drastically with age, such that it had the largest crimp in neonates, and among the smallest in the elderly. Age-related decreases in crimp parameters are likely one of the mechanisms underlying age-related stiffening of the sclera and cornea, potentia...Continue Reading

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Citations

Oct 30, 2018·Investigative Ophthalmology & Visual Science·Bryn L BrazileIan A Sigal
Jun 20, 2019·Biochemistry. Biokhimii︠a︡·Yu V KistenevN A Krivova
Aug 15, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yuening Shen
Oct 12, 2018·Investigative Ophthalmology & Visual Science·Alexandra GogolaIan A Sigal
Nov 10, 2020·Investigative Ophthalmology & Visual Science·Andrew P VoorheesIan A Sigal
Aug 15, 2019·Progress in Retinal and Eye Research·Craig BooteMichael J A Girard
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Mar 27, 2020·Current Opinion in Biomedical Engineering·Rafael GrytzIan A Sigal
May 23, 2021·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Marcelina SobczakBarbara Pierścionek
Aug 25, 2021·The American Journal of Pathology·Xinwei JiaoZhijie Li
Dec 1, 2021·Journal of Cataract and Refractive Surgery·Vikas MittalNancy Sehdev
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yongfang XieGuohui Wang

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Methods Mentioned

BETA
light microscopy
X-ray
imaging techniques
light scattering

Software Mentioned

Fiji Is Just ImageJ
FIJI

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