Spatial Patterns of Rhizoplane Populations of Pseudomonas fluorescens.

Applied and Environmental Microbiology
L M DandurandG R Knudsen

Abstract

Geostatistical analysis was used to compare rhizoplane colonization patterns of an antibiotic-producing biological control bacterium versus a non-antibiotic-producing mutant strain. Pea seeds were inoculated with Pseudomonas fluorescens 2-79RN(inf10) or P. fluorescens 2-79-B46 (a phenazine-deficient Tn5 mutant of P. fluorescens 2-79RN(inf10)) (10(sup8) CFU/pea), planted in sterile sand, and incubated at 20(deg)C. After 3 days, seedlings were prepared for scanning electron microscopy. Photomicrographs (x1,000) of the root surface were taken at the seed-root junction and at 0.5-cm intervals to the root tip. Bacterial counts on the root surface were made in 5- by 5-(mu)m sample units over an area which was 105 by 80 (mu)m. Coordinates and number of bacteria were recorded for each sample unit. Spatial statistics were calculated by covariance for the following directions: omnidirectional, 0, 45, 90, and 135(deg). The ranges of spatial influence and nugget (estimator of spatially dependent variation) were determined. For both P. fluorescens 2-79RN(inf10) and P. fluorescens 2-79-B46, spatial structure was evident along the entire root, particularly in the 0(deg) direction (along the root length) (e.g., range = 24 (mu)m, nugget = 0.52)...Continue Reading

References

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Citations

Nov 10, 2001·Annual Review of Phytopathology·John H Andrews, Robin F Harris
May 30, 2006·Current Microbiology·Padma Sudarshana, Guy R Knudsen
May 31, 2013·The ISME Journal·Vanessa L BaileyLee Ann McCue
May 6, 2003·Annual Review of Phytopathology·Cindy E Morris, Jean-Michel Monier
Dec 23, 2011·Canadian Journal of Microbiology·Caressa J CaldwellDarren R Korber
May 18, 2007·Annual Review of Microbiology·Thomas Danhorn, Clay Fuqua
Mar 13, 2015·FEMS Microbiology Ecology·Daniel S EsserKerstin Wiegand

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