Spatial positioning of all 24 chromosomes in the lymphocytes of six subjects: evidence of reproducible positioning and spatial repositioning following DNA damage with hydrogen peroxide and ultraviolet B

PloS One
Dimitrios IoannouH G Tempest

Abstract

The higher-order organization of chromatin is well-established, with chromosomes occupying distinct positions within the interphase nucleus. Chromatin is susceptible to, and constantly assaulted by both endogenous and exogenous threats. However, the effects of DNA damage on the spatial topology of chromosomes are hitherto, poorly understood. This study investigates the organization of all 24 human chromosomes in lymphocytes from six individuals prior to- and following in-vitro exposure to genotoxic agents: hydrogen peroxide and ultraviolet B. This study is the first to report reproducible distinct hierarchical radial organization of chromosomes with little inter-individual differences between subjects. Perturbed nuclear organization was observed following genotoxic exposure for both agents; however a greater effect was observed for hydrogen peroxide including: 1) More peripheral radial organization; 2) Alterations in the global distribution of chromosomes; and 3) More events of chromosome repositioning (18 events involving 10 chromosomes vs. 11 events involving 9 chromosomes for hydrogen peroxide and ultraviolet B respectively). Evidence is provided of chromosome repositioning and altered nuclear organization following in-vitro...Continue Reading

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Citations

Jul 7, 2015·Molecular Cytogenetics·Dimitrios IoannouHelen Ghislaine Tempest
Jan 3, 2018·Systems Biology in Reproductive Medicine·Dimitrios Ioannou, Helen G Tempest
Jul 3, 2021·International Journal of Molecular Sciences·Atiqa SajidMohammed Yusuf
Sep 1, 2021·Cellular and Molecular Life Sciences : CMLS·Stefano AmenteMarcus S Cooke

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BETA
chromosomal aberrations
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