Spatial Regulation of Biomolecular Interactions with a Switchable Trident-Shaped DNA Nanoactuator

ACS Applied Materials & Interfaces
Chao XingHuang-Hao Yang

Abstract

DNA nanostructures with controllable motions and functions have been used as flexible scaffolds to precisely and spatially organize molecular reactions at the nanoscale. The construction of dynamic DNA nanostructures with site-specifically incorporated functional elements is a critical step toward building nanomachines. Artificial self-assembled DNA nanostructures have also been developed to mimic key biological processes like various small biomolecule- and protein-based functional biochemistry pathways. Here, we report a self-assembled dynamic trident-shaped DNA (TS DNA) nanoactuator, in which biomolecules can be tethered to the three "arms" of the TS DNA nanoactuator. The TS DNA nanoactuator is implemented as the mechanical scaffold for the reconfiguration of fluorescent/quenching molecules and the assembly of gold nanoparticles, which exhibit controlled spatial separation. Furthermore, two enzymes (glucose oxidase and horseradish peroxidase) are attached to the two outer arms of the TS DNA nanoactuator, which show an enhanced cascade reaction efficiency compared to free enzymes. The efficiency of the two-enzyme cascade reaction can be spatially regulated by switching the TS DNA nanoactuator between opened, semiopened, and cl...Continue Reading

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Citations

Apr 6, 2020·Topics in Current Chemistry·Jinglin FuTing Zhang
Feb 10, 2021·ACS Applied Materials & Interfaces·Manman HeXia Chu
Mar 10, 2020·Analytical Chemistry·Jinjie LiHeyou Han

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