Spatial regulation of organelle release from myosin V transport by p21-activated kinases.

The Journal of Cell Biology
Richard G YauL S Weisman

Abstract

Correct positioning of organelles is essential to eukaryotic cells. Molecular motors transport organelles to their proper destinations, yet little is known about the pathways that define these destinations. In Saccharomyces cerevisiae, the myosin V motor Myo2 binds the vacuole-specific adapter Vac17 to attach to the vacuole/lysosome and initiate transport. After arrival in the bud, Myo2 releases the vacuole, and Vac17 is degraded. However, the mechanisms that spatially regulate this release were not established. In this study, we report that the bud cortex is a landmark that signals a successful delivery of the vacuole to the bud. We demonstrate that upon arrival at the bud cortex, Vac17 is phosphorylated by Cla4. Cla4-dependent phosphorylation is required for the ubiquitylation and subsequent degradation of Vac17 and the release of the vacuole from Myo2. Our study reveals a critical step in the spatial regulation of myosin V-dependent organelle transport and may reveal common mechanisms for how molecular motors accurately deposit cargoes at the correct locations.

References

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Citations

Aug 17, 2018·Journal of Cell Science·Stefanie J OedingMartin Bähler
Feb 6, 2021·Advances in Biological Regulation·Sara Wong, Lois S Weisman
Jun 11, 2021·Current Genetics·Sara Wong, Lois S Weisman

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Methods Mentioned

BETA
nucleotide exchange
immunoprecipitation
Fluorescence microscopy
immunodepletion
affinity purification

Software Mentioned

ImageJ
FIJI
Photoshop

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