Spatial specificity of auxin responses coordinates wood formation

Nature Communications
Klaus BrackmannThomas Greb

Abstract

Spatial organization of signalling events of the phytohormone auxin is fundamental for maintaining a dynamic transition from plant stem cells to differentiated descendants. The cambium, the stem cell niche mediating wood formation, fundamentally depends on auxin signalling but its exact role and spatial organization is obscure. Here we show that, while auxin signalling levels increase in differentiating cambium descendants, a moderate level of signalling in cambial stem cells is essential for cambium activity. We identify the auxin-dependent transcription factor ARF5/MONOPTEROS to cell-autonomously restrict the number of stem cells by directly attenuating the activity of the stem cell-promoting WOX4 gene. In contrast, ARF3 and ARF4 function as cambium activators in a redundant fashion from outside of WOX4-expressing cells. Our results reveal an influence of auxin signalling on distinct cambium features by specific signalling components and allow the conceptual integration of plant stem cell systems with distinct anatomies.

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Citations

Oct 9, 2019·Nature Plants·Thomas Greb
Mar 21, 2020·Cellular and Molecular Life Sciences : CMLS·Javier Agustí, Miguel A Blázquez
Mar 2, 2019·Annual Review of Plant Biology·Urs FischerRishikesh P Bhalerao
Apr 3, 2020·Plant Signaling & Behavior·Bhavani Natarajan, Anjan K Banerjee
Dec 16, 2018·Plants·Eva HellmannYkä Helariutta
Jul 4, 2018·Planta·Marc BehrGea Guerriero
Feb 24, 2021·Journal of Experimental Botany·Mehdi Ben-TargemLaura Ragni
Jan 14, 2020·Plant Science : an International Journal of Experimental Plant Biology·Huanzhong Wang
Jun 16, 2021·Current Biology : CB·Lingling YeAri Pekka Mähönen
Apr 28, 2021·Cold Spring Harbor Perspectives in Biology·Yanfei MaJan U Lohmann
Oct 17, 2021·Journal of Experimental Botany·Emma K Turley, J Peter Etchells

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Datasets Mentioned

BETA
GSE98193

Methods Mentioned

BETA
electrophoretic mobility shift assays
PCR
transgenic
Assay
electrophoresis
Fluorescence

Software Mentioned

ImageJ2
VirtualPlant
Bioconductor
SPSS Statistics
Zen
Excel
CLC Genomics Workbench
R
ChemoStar Professional
3DHistech

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