Spatial (Tbata) expression in mature medullary thymic epithelial cells

European Journal of Immunology
Murielle SaadeCatherine Nguyen

Abstract

The Spatial gene is expressed in highly polarized cell types such as testis germ cells, brain neurons and thymic epithelial cells (TEC). Its expression was documented in testis and brain but poorly characterized in thymus. Here, we characterize for the first time Spatial-expressing TEC throughout ontogeny and adult mouse thymus. Spatial is expressed in thymic-fated domain by embryonic day E10.5 and persists in subcapsular, cortical, medullary epithelial cells and in MTS24(+) progenitor TEC. Using mouse strains in which thymocyte development is blocked at various stages, we show that Spatial expression is independent of thymocyte-derived signals during thymus organogenesis. Analyses on purified thymic cell subsets show that Spatial short isoforms are expressed in cortical TEC (cTEC) and mature medullary TEC (mTEC). Spatial long isoforms were detected in the same TEC population. Spatial presents a nuclear distribution specific to mature mTEC expressing UEA1 and Aire. Aire- and RANKL-deficient mice revealed that Spatial expression is drastically reduced in the thymus of these mutants. These findings reveal a critical function of Aire in regulating Spatial expression, which is compatible with promiscuous Spatial gene expression.

References

Sep 1, 1993·Immunology Today·R L BoydP Hugo
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·C C BlackburnG Morahan
Aug 1, 1997·Microscopy Research and Technique·B Von GaudeckerM A Ritter
Sep 29, 1999·Genes & Development·W C DougallJ Schuh
Feb 21, 2002·Human Molecular Genetics·Chris RamseyLeena Peltonen
Mar 22, 2002·Nature Reviews. Immunology·G Anderson, E J Jenkinson
Jul 18, 2002·Immunity·Andrea R BennettC Clare Blackburn
Jul 23, 2002·Trends in Immunology·C Clare BlackburnMary A Ritter
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·David B KlugEllen R Richie
Mar 14, 2003·Current Opinion in Immunology·Nancy R Manley, C Clare Blackburn
Apr 25, 2003·Gene Expression Patterns : GEP·Magali IrlaCatherine Nguyen
Sep 3, 2004·Nature Reviews. Immunology·Bruno Kyewski, Jens Derbinski
Jun 29, 2005·The Journal of Experimental Medicine·Geoffrey O Gillard, Andrew G Farr
Aug 23, 2005·Immunity·Mark S AndersonDiane Mathis
Mar 23, 2006·Annual Review of Immunology·Bruno Kyewski, Ludger Klein
Jan 2, 2007·Experimental Cell Research·Murielle SaadeCatherine Nguyen
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geoffrey O GillardAndrew G Farr
Aug 19, 2007·European Journal of Immunology·Simona W RossiGraham Anderson
Oct 3, 2007·The Journal of Experimental Medicine·Tom M McCaughtryKristin A Hogquist
Nov 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juan LiWei-Feng Chen
Jan 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jens DerbinskiBruno Kyewski
Apr 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Natalie SeachRichard L Boyd
Mar 24, 2009·Annual Review of Immunology·Diane Mathis, Christophe Benoist

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Citations

Jan 5, 2011·Current Opinion in Immunology·Takeshi NittaYousuke Takahama
Aug 17, 2016·Current Stem Cell Reports·Asako TajimaYong Fan
Dec 24, 2013·Molecular and Cellular Neurosciences·Miriam YammineCatherine Nguyen
Sep 14, 2013·Journal of Proteomics·Gabriel EspinosaIñaki Alvarez

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