Spatial uncoupling of mitosis and cytokinesis during appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae.

The Plant Cell
Diane G O SaundersN J Talbot

Abstract

To infect plants, many pathogenic fungi develop specialized infection structures called appressoria. Here, we report that appressorium development in the rice blast fungus Magnaporthe oryzae involves an unusual cell division, in which nuclear division is spatially uncoupled from the site of cytokinesis and septum formation. The position of the appressorium septum is defined prior to mitosis by formation of a heteromeric septin ring complex, which was visualized by spatial localization of Septin4:green fluorescent protein (GFP) and Septin5:GFP fusion proteins. Mitosis in the fungal germ tube is followed by long-distance nuclear migration and rapid formation of an actomyosin contractile ring in the neck of the developing appressorium, at a position previously marked by the septin complex. By contrast, mutants impaired in appressorium development, such as Deltapmk1 and DeltacpkA regulatory mutants, undergo coupled mitosis and cytokinesis within the germ tube. Perturbation of the spatial control of septation, by conditional mutation of the SEPTATION-ASSOCIATED1 gene of M. oryzae, prevented the fungus from causing rice blast disease. Overexpression of SEP1 did not affect septation during appressorium formation, but instead led to de...Continue Reading

References

Jan 1, 1993·Cell Motility and the Cytoskeleton·A J KoningR Wright
Jan 15, 1997·Nucleic Acids Research·K R OldenburgC Paddon
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·H O ParkI Herskowitz
Sep 10, 1998·The Journal of Cell Biology·E BiJ R Pringle
Dec 16, 1998·The Journal of Cell Biology·J BählerD McCollum
Feb 9, 2000·Molecular Cell Biology Research Communications : MCBRC·R CenamorM Sánchez
Feb 15, 2001·Fungal Genetics and Biology : FG & B·S G Kaminskyj
Nov 10, 2001·Annual Review of Phytopathology·S L Tucker, N J Talbot
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·A J Bardin, A Amon
Dec 4, 2001·Current Opinion in Microbiology·S D Harris
Jan 26, 2002·Molecular Biology of the Cell·Patrick J Westfall, Michelle Momany
Apr 6, 2002·Current Opinion in Microbiology·Antonio Casamayor, Michael Snyder
Apr 16, 2002·Molecular Plant-microbe Interactions : MPMI·Gyungsoon ParkJin-Rong Xu
Dec 4, 2003·Current Opinion in Cell Biology·Daniel J Lew
Jan 1, 1997·Annual Review of Phytopathology·R A Dean
May 25, 2004·Molecular Biology of the Cell·Claire L PearsonSteven D Harris
May 25, 2004·The Plant Cell·Sara L TuckerNicholas J Talbot
Mar 24, 2005·Journal of Cell Science·Amy S GladfelterDaniel J Lew
Sep 10, 2005·Eukaryotic Cell·Lois M DouglasJames B Konopka
Feb 2, 2006·The Journal of Cell Biology·Amy S GladfelterPeter Philippsen
Apr 29, 2006·Science·Claire Veneault-FourreyNicholas J Talbot
Jul 22, 2006·Microbiology and Immunology·Alberto González-NovoJavier Jiménez
Feb 17, 2009·Nature Reviews. Microbiology·Richard A Wilson, Nicholas J Talbot
Mar 21, 2009·Genes & Development·Snezhana OliferenkoMohan K Balasubramanian
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael J Kershaw, Nicholas J Talbot
Nov 10, 2009·Nature Reviews. Microbiology·Amy Gladfelter, Judith Berman
Mar 2, 2010·The Plant Cell·Diane G O SaundersNicholas J Talbot

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Citations

Jun 23, 2012·Science·Yasin F DagdasNicholas J Talbot
Jul 9, 2013·Bioscience, Biotechnology, and Biochemistry·Akira IshiiTakashi Kamakura
Jan 26, 2016·Microbiological Research·Mauricio Batista FialhoSérgio Florentino Pascholati
Jun 7, 2011·Fungal Biology·Alexander LichiusNick D Read
Apr 2, 2016·Seminars in Cell & Developmental Biology·José Pérez-MartínMaría Tenorio-Gómez
Apr 4, 2016·Seminars in Cell & Developmental Biology·Robert Marschall, Paul Tudzynski
Jun 5, 2015·Current Opinion in Plant Biology·Lauren S Ryder, Nicholas J Talbot
Oct 12, 2010·Current Opinion in Microbiology·Amy S Gladfelter
Aug 16, 2011·Fungal Genetics and Biology : FG & B·Leonie KokkelinkPaul Tudzynski
Jun 21, 2016·Fungal Genetics and Biology : FG & B·Kiersun JonesChang Hyun Khang
Sep 16, 2016·Molecular Biology of the Cell·Mahesh ChemudupatiStephen A Osmani
Mar 1, 2017·Environmental Microbiology·Guotian LiJin-Rong Xu
Jul 15, 2017·Molecular & Cellular Proteomics : MCP·Rui-Jin WangYou-Liang Peng
Dec 21, 2017·Communicative & Integrative Biology·Míriam Osés-Ruiz, Nicholas J Talbot
Mar 3, 2015·The New Phytologist·Diane G O Saunders
Oct 22, 2015·Annual Review of Microbiology·Anum KhanAmy S Gladfelter

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