Spatial variation in the molecular tilt orientational order within the solid domains of phase-separated, mixed dialkylphosphatidylcholine monolayers

Chemistry and Physics of Lipids
Jacqueline Sanchez, Antonella Badia

Abstract

The miscibility of the solid-phase-forming distearoylphosphatidylcholine (DSPC) and the fluid-phase-forming dilauroylphosphatidylcholine (DLPC) at the air/water interface was investigated by the Langmuir film balance. Surface pressure-area isotherms suggest that mixtures containing 25.0-62.5-mol% DLPC (range of composition investigated) are phase-separated. The lateral structure of the DSPC/DLPC monolayers was imaged by Brewster angle microscopy (BAM) as a function of the surface pressure. Quasi-circular condensed domains appeared at pressures between 0 and 0.5mN m(-1), and these structures were already fully developed at approximately 1mN m(-1). Further compression of the monolayers above 1mN m(-1) merely brought the domains closer together. The mixed monolayers consisted of solid domains of DSPC, approximately 3-20 micro in size, in a fluid matrix of DLPC. BAM and the phase contrast mode of intermittent-contact atomic force microscopy (AFM) revealed that the quasi-circular DSPC domains are divided into segments of different reflectivities (BAM) or phase shift (AFM) that arise from abrupt changes in the long-range orientational order of the tilted hydrocarbon chains. The DSPC domains in DSPC/DLPC internally exhibited star and ...Continue Reading

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Citations

Dec 17, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Edward S AllgeyerMichael D Mason
Apr 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Paweł WydroMarta Lapczyńska
Nov 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sabina Tatur, Antonella Badia
Jan 17, 2021·Membranes·Anna Chachaj-BrekieszPatrycja Dynarowicz-Latka
Oct 7, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Nathalie Y-Wa Tang, Antonella Badia
Sep 30, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Dayane S AlvaresNatalia Wilke
Oct 14, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Laurianne Gravel-TattaAntonella Badia

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