Spatially dynamic intercellular adhesion junction is coupled to a microtubule-based motility system: evidence from an in vitro binding assay

Cell Motility and the Cytoskeleton
A W Vogl

Abstract

During spermatogenesis, spermatids change position in the seminiferous epithelium along an axis that is perpendicular to the seminiferous tubule wall. During this period, spermatids are attached to apical invaginations of Sertoli cells. In areas of this attachment, unique junction plaques occur in Sertoli cells. These plaques consist of regions of the plasma membrane involved with intercellular adhesion, a layer of actin filaments that are hexagonally packed, and an underlying cistern of endoplasmic reticulum (ER). It previously has been proposed that these junction plaques, and therefore the attached spermatids, are translocated, by motor proteins, along microtubule tracts in the Sertoli cell. If this is true, microtubules should bind to the junction plaque in a nucleotide dependent fashion. To verify this prediction, seminiferous epithelia of the rat were separated from tubule walls and then mechanically fragmented. These epithelial preparations were incubated, in both the presence and absence of 10 mM Mg(+)(+)ATP, with exogenous microtubules stabilized with taxol. Then unbound microtubules were separated from microtubules bound to larger epithelial components by centrifuging the samples through a step sucrose gradient. The f...Continue Reading

References

Dec 1, 1992·Biology of Reproduction·F PalombiM Stefanini
Jan 1, 1991·Annals of the New York Academy of Sciences·A W VoglD M Redenbach
Aug 1, 1988·International Journal of Andrology·C A Suárez-Quian, M Dym
Jan 1, 1967·Zeitschrift für Zellforschung und mikroskopische Anatomie·L Nicander
Jan 1, 1984·Annals of the New York Academy of Sciences·C A Suárez-Quian, M Dym
Jun 1, 1994·The Journal of Cell Biology·S KondoN Hirokawa
Feb 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·E S HallK Boekelheide
Feb 1, 1995·Current Opinion in Cell Biology·N B Cole, J Lippincott-Schwartz
Jan 1, 1963·Zeitschrift für Zellforschung und mikroskopische Anatomie·J BROKELMANN

❮ Previous
Next ❯

Citations

Apr 27, 2001·Biochemical and Biophysical Research Communications·A M Zambito, J Wolff
Apr 19, 2000·Archives of Histology and Cytology·A W VoglJ Guttman
May 26, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Julian A GuttmanA Wayne Vogl
Nov 30, 2006·Journal of Cellular Physiology·K S VaidA W Vogl
Nov 3, 2004·Trends in Endocrinology and Metabolism : TEM·Dolores D Mruk, C Yan Cheng
May 31, 2016·Biological Trace Element Research·Sabah AnsarMukhtar M Aleem
Feb 9, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Arlo Adams, A Wayne Vogl
Sep 15, 2007·Biology of Reproduction·Kuljeet S VaidA Wayne Vogl
Sep 12, 2015·Endocrine Reviews·Dolores D Mruk, C Yan Cheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.