Spatially modulated illumination microscopy allows axial distance resolution in the nanometer range

Applied Optics
Benno AlbrechtChristoph Cremer

Abstract

For an improved understanding of the structural basis of cellular mechanisms, it is highly desirable to develop methods for a detailed topological analysis of biological nanostructures and their dynamics in the interior of three-dimensionally conserved cells. We present a method of far-field laser fluorescence microscopy to measure relative axial positions of pointlike fluorescent targets and the distance between each target in the range of a few nanometers. The physical principle behind this approach can be extended to the determination of three-dimensional (3D) positions and 3D distances between any number of objects that can be discriminated owing to their spectral signature, thus allowing topological measurements so far regarded to be beyond the capabilities of light microscopy.

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R LakowiczM L Johnson
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·T CremerP Lichter
Apr 1, 1996·Nature Genetics·M R SpeicherD C Ward
Jul 26, 1996·Science·E SchröckT Ried
May 9, 1998·Science·A I Lamond, W C Earnshaw
Sep 24, 1998·Biophysical Journal·M SchraderS W Hell
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·T A KlarS W Hell
Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·T D LacosteS Weiss
Sep 28, 2000·Current Opinion in Chemical Biology·D M Lilley, T J Wilson
Sep 28, 2000·Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology·R HeintzmannC Cremer
Apr 3, 2001·Nature Reviews. Genetics·T Cremer, C Cremer

❮ Previous
Next ❯

Citations

Mar 13, 2014·Histochemistry and Cell Biology·C CremerU Birk
Mar 17, 2004·Molecular Biology of the Cell·Sonya MartinAna Pombo
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kareem ElsayadKatrin G Heinze
May 8, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jürgen ReymannUdo Birk
Nov 15, 2005·Histochemistry and Cell Biology·H MathéeU Birk
Jul 21, 2010·The Journal of Cell Biology·Lothar SchermellehHeinrich Leonhardt
Dec 17, 2011·Micron : the International Research and Review Journal for Microscopy·Lindsy M RapkinDavid P Bazett-Jones
Jun 16, 2006·Journal of Microscopy·J BewersdorfS W Hell
Sep 13, 2011·Biotechnology Journal·Christoph CremerMichael Hausmann
Oct 19, 2006·Journal of Structural Biology·Rebecca MeddaJörg Bewersdorf
Dec 3, 2014·Ultramicroscopy·Giulio GuzzinatiJo Verbeeck
Nov 28, 2007·Differentiation; Research in Biological Diversity·Joachim RauchChristoph Cremer
Dec 13, 2002·Applied Optics·Antonio Virgillo FaillaChristoph Cremer
Oct 20, 2007·Nature Protocols·David BaddeleyUdo J Birk
Jul 17, 2008·Optics Letters·Pedro Felipe Gardeazábal RodríguezVincent Loriette
Nov 5, 2002·Applied Optics·Antonio Virgilio FaillaChristoph Cremer
May 14, 2003·Genome Research·Timothy P O'BrienRoel van Driel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.