Spatially pathogenic forms of tau detected in Alzheimer's disease brain tissue by fluorescence lifetime-based Förster resonance energy transfer

Journal of Neuroscience Methods
Sergey LarionovHarald Neumann

Abstract

In tauopathies including Alzheimer's disease (AD) tau molecules have lost their normal spatial distance to each other and appear in oligomeric or aggregated forms. Conventional immunostaining methods allow detection of abnormally phosphorylated or conformationally altered aggregated tau proteins, but fail to visualize oligomeric forms of tau. Here we show that tau molecules that lost their normal spatial localization can be detected on a subcellular level in postmortem central nervous system (CNS) tissue sections of AD patients by fluorescence lifetime-based Förster resonance energy transfer (FRET). Paraffin sections were co-immunostained with two tau-specific monoclonal antibodies recognizing the same epitope, but labeled with distinct fluorescence dyes suitable for spatial resolution at a nanometer scale by lifetime-based FRET. A FRET signal was detected in neuritic plaques and neurofibrillary tangles of CNS tissue sections of AD patients, showing associated tau proteins typically reflecting either fibrillary, oligomeric or aggregated tau. The 'pretangle-like' structures within the neuronal perikarya did not contain spatially pathogenic forms of tau accordingly to this method. Data demonstrate that fluorescence lifetime-based...Continue Reading

References

Jan 1, 1991·Acta Neuropathologica·H Braak, E Braak
Jan 1, 1990·Methods in Enzymology·N M Green
Jan 1, 1989·Acta Neuropathologica·H M WisniewskiK S Kim
Oct 1, 1988·The Journal of Cell Biology·N HirokawaS Okabe
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·M G SpillantiniB Ghetti
Nov 13, 1998·Current Opinion in Neurobiology·M GoedertS W Davies
Dec 17, 1998·Trends in Cell Biology·E M Mandelkow, E Mandelkow
Jul 19, 2000·Biochimica Et Biophysica Acta·P FriedhoffE Mandelkow
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·D Leckband
Aug 22, 2000·Journal of Neuropathology and Experimental Neurology·D R ThalH Braak
Aug 25, 2001·Annual Review of Neuroscience·V M LeeJ Q Trojanowski
Jun 1, 1964·Brain : a Journal of Neurology·M KIDD
Dec 17, 2003·Microscopy Research and Technique·Ye Chen, Ammasi Periasamy
Dec 23, 2003·Differentiation; Research in Biological Diversity·Ye ChenAmmasi Periasamy
Jun 9, 2004·Biology of the Cell·Marion Peter, Simon M Ameer-Beg
Oct 21, 2004·The Journal of Biological Chemistry·Anja SchneiderMikael Simons
Dec 24, 2004·Biochimica Et Biophysica Acta·Khalid IqbalInge Grundke-Iqbal
Dec 24, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Klaus SuhlingDavid Phillips
Apr 29, 2005·Brain : a Journal of Neurology·Yang LiuKlaus Fassbender
Nov 22, 2005·Nature Methods·Nathan C ShanerRoger Y Tsien
Aug 10, 2006·Journal of Neuropathology and Experimental Neurology·Irina AlafuzoffHans Kretzschmar
Oct 28, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Massimiliano StagiHarald Neumann
Sep 4, 2007·Trends in Biochemical Sciences·David W Piston, Gert-Jan Kremers
Apr 1, 2008·Methods : a Companion to Methods in Enzymology·Anne V ThomasChristine A F von Arnim
Jun 24, 2008·Nature Reviews. Neuroscience·Jürgen Götz, Lars M Ittner
Jul 8, 2008·Current Opinion in Biotechnology·Francisco Ciruela
Jan 3, 2009·Methods in Cell Biology·Ammasi PeriasamyMargarida Barroso
Feb 3, 2009·Acta Neuropathologica·Khalid IqbalInge Grundke-Iqbal
Mar 10, 2009·Current Opinion in Biotechnology·James A LevittKlaus Suhling
Mar 18, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Philipp GorlovoyHarald Neumann

❮ Previous
Next ❯

Citations

Jul 3, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yuansheng SunAmmasi Periasamy
Oct 15, 2010·International Journal of Neural Systems·Jose Antonio IglesiasAraceli Sanchis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Alzheimer's Disease: Tau & TDP-43

Alzheimer's disease is a neurodegenerative disease. This feed focuses on the underlying role of tau proteins and TAR DNA-binding protein 43, as well as other genetic factors, in Alzheimer's disease.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Philipp GorlovoyHarald Neumann
Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Ian R MacdonaldSultan Darvesh
© 2021 Meta ULC. All rights reserved