Spatially propagating activation of quorum sensing in Vibrio fischeri and the transition to low population density.

Physical Review. E
Keval PatelStephen J Hagen

Abstract

Bacteria communicate by secreting and detecting diffusible small molecule signals or pheromones. Using the local concentrations of these signals to regulate gene expression, individual cells can synchronize changes in phenotype population-wide, a behavior known as quorum sensing (QS). In unstirred media, the interplay between diffusion of signals, bacterial growth, and regulatory feedback can generate complex spatial and temporal patterns of expression of QS-controlled genes. Here we identify the parameters that allow a local signal to trigger a self-sustaining, traveling activation of QS behavior. Using the natural bioluminescence of wild-type Vibrio fischeri as a readout of its lux QS system, we measure the induction of a spreading QS response by a localized triggering stimulus in unstirred media. Our data show that a QS response propagates outward, sustained by positive feedback in synthesis of the diffusible signal, and that this response occurs only if the triggering stimulus exceeds a critical threshold. We also test how the autonomous or untriggered activation of the V. fischeri QS pathway changes at very low initial population densities. At the lowest population densities, clusters of cells do not transition to a self-s...Continue Reading

References

Sep 1, 1985·Journal of Bacteriology·H B Kaplan, E P Greenberg
Mar 1, 1972·Journal of Bacteriology·A Eberhard
Oct 1, 1970·Journal of Bacteriology·K H NealsonJ W Hastings
Dec 12, 2002·Methods in Enzymology·Eric V Stabb, Edward G Ruby
Sep 6, 2006·Analytical and Bioanalytical Chemistry·Anne K Dunn, Eric V Stabb
Feb 17, 2007·Nature Reviews. Microbiology·Burkhard A HenseJan-Ulrich Kreft
Aug 19, 2009·Annual Review of Genetics·Wai-Leung Ng, Bonnie L Bassler
Nov 26, 2009·Nature Chemical Biology·Eric C CarnesC Jeffrey Brinker
Jan 22, 2010·Nature·Tal DaninoJeff Hasty
Jun 30, 2010·PLoS Computational Biology·Pontus MelkeHenrik Jönsson
Jun 2, 2011·Journal of Biological Physics·Stephen J HagenJonathan H Young
Mar 1, 2012·Journal of the American Chemical Society·Gabriel E DilanjiStephen J Hagen
May 18, 2012·Current Opinion in Chemical Biology·John S Chuang
Jun 6, 2012·Sensors·Burkhard A HenseAnton Hartmann
Aug 24, 2013·International Journal of Molecular Sciences·Subhash C Verma, Tim Miyashiro
Sep 24, 2013·Journal of Bacteriology·John H Kimbrough, Eric V Stabb
Aug 12, 2014·Journal of Theoretical Biology·Jessica B LangebrakePatrick De Leenheer
Nov 20, 2014·The Open Microbiology Journal·Jakob M Ondrey, Karen L Visick
Apr 14, 2016·PLoS Computational Biology·David N QuanWilliam E Bentley
Aug 12, 2016·Nature Reviews. Microbiology·Kai Papenfort, Bonnie L Bassler
Oct 5, 2016·Scientific Reports·Meng GaoXiaojun Ma
May 13, 2018·Applied and Environmental Microbiology·Julie L StoudenmireEric V Stabb
Nov 6, 2018·Chaos·Vera BettenworthBruno Eckhardt
May 22, 2019·Physical Review. E·Kalinga Pavan T SilvaJames Q Boedicker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Hospital Topics
Barbara Hutt Clarke
Organic & Biomolecular Chemistry
Thanit PraneenararatHelen E Blackwell
Expert Opinion on Therapeutic Patents
Tianyu Jiang, Minyong Li
© 2021 Meta ULC. All rights reserved