Spatially remote motifs cooperatively affect substrate preference of a ruminal GH26-type endo-β-1,4-mannanase.

The Journal of Biological Chemistry
Fernanda MandelliMário Tyago Murakami

Abstract

β-Mannanases from the glycoside hydrolase 26 (GH26) family are retaining hydrolases that are active on complex heteromannans and whose genes are abundant in rumen metagenomes and metatranscriptomes. These enzymes can exhibit distinct modes of substrate recognition and are often fused to carbohydrate-binding modules (CBMs), resulting in a molecular puzzle of mechanisms governing substrate preference and mode of action that has not yet been pieced together. In this study, we recovered a novel GH26 enzyme with a CBM35 module linked to its N terminus (CrMan26) from a cattle rumen metatranscriptome. CrMan26 exhibited a preference for galactomannan as substrate and the crystal structure of the full-length protein at 1.85 Å resolution revealed a unique orientation of the ancillary domain relative to the catalytic interface, strategically positioning a surface aromatic cluster of the ancillary domain as an extension of the substrate-binding cleft, contributing to galactomannan preference. Moreover, systematic investigation of nonconserved residues in the catalytic interface unveiled that residues Tyr195 (-3 subsite) and Trp234 (-5 subsite) from distal negative subsites have a key role in galactomannan preference. These results indicate...Continue Reading

References

Oct 1, 1981·European Journal of Biochemistry·P BielyM Vrsanská
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
May 16, 2009·Biochemistry·Louise E TailfordHarry J Gilbert
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Mar 13, 2010·PloS One·Morgan N PriceAdam P Arkin
Jan 29, 2011·Science·Matthias HessEdward M Rubin
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
May 26, 2012·Science·P Andrew Karplus, Kay Diederichs
Nov 26, 2013·Nucleic Acids Research·Vincent LombardBernard Henrissat
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
May 1, 2016·Nucleic Acids Research·Liisa Holm, Laura M Laakso
Sep 7, 2017·Protein Science : a Publication of the Protein Society·Roman A LaskowskiJanet M Thornton
May 4, 2018·Bioinformatics·Nirvana NursimuluJohn Parkinson
May 18, 2018·Nucleic Acids Research·Han ZhangYanbin Yin
Jul 22, 2018·Biotechnology for Biofuels·Pernille von FreieslebenAnne S Meyer
Apr 13, 2019·Nucleic Acids Research·Fábio MadeiraRodrigo Lopez
Apr 20, 2019·The Journal of Biological Chemistry·Viktoria BågenholmHenrik Stålbrand
May 6, 2019·FEMS Microbiology Reviews·Sarah Moraïs, Itzhak Mizrahi

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Jun 18, 2021·Frontiers in Chemistry·Xiaoxiao WangLiming Zhao

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