Spatially Resolved Analytical Chemistry in Intact, Living Tissues.

Analytical Chemistry
Maura C BelangerRebecca R Pompano

Abstract

Tissues are an exciting frontier for bioanalytical chemistry, one in which spatial distribution is just as important as total content. Intact tissue preserves the native cellular and molecular organization and the cell-cell contacts found in vivo. Live tissue, in particular, offers the potential to analyze dynamic events in a spatially resolved manner, leading to fundamental biological insights and translational discoveries. In this Perspective, we provide a tutorial on the four fundamental challenges for the bioanalytical chemist working in living tissue samples as well as best practices for mitigating them. The challenges include (i) the complexity of the sample matrix, which contributes myriad interfering species and causes nonspecific binding of reagents; (ii) hindered delivery and mixing; (iii) the need to maintain physiological conditions; and (iv) tissue reactivity. This framework is relevant to a variety of methods for spatially resolved chemical analysis, including optical imaging, inserted sensors and probes such as electrodes, and surface analyses such as sensing arrays. The discussion focuses primarily on ex vivo tissues, though many considerations are relevant in vivo as well. Our goal is to convey the exciting pot...Continue Reading

References

Aug 11, 1982·Biochimica Et Biophysica Acta·J Slavík
Jul 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C H Van de LestT H Van Kuppevelt
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·K Francis, B O Palsson
Jun 4, 1998·Trends in Neurosciences·C Nicholson, E Syková
Nov 14, 1998·Biochimica Et Biophysica Acta·K KachelE London
Nov 26, 1999·Immunology and Cell Biology·C R Parish
Jun 7, 2000·The Journal of Clinical Investigation·C Booth, C S Potten
Jul 12, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jonathan V SehyJeffrey J Neil
Jul 13, 2002·The Journal of Comparative Neurology·Aline M DavisStuart A Tobet
Oct 29, 2003·Biophysical Journal·Alexey N BashkatovValery V Tuchin
Feb 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Stephan ThibergeElisha Moses
Feb 17, 2005·JAMA : the Journal of the American Medical Association·Farouc A Jaffer, Ralph Weissleder
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Linda G Griffith, Melody A Swartz
Mar 7, 2006·Journal of Biochemical and Biophysical Methods·Yufen ZhangStefan Balaz
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert G Thorne, Charles Nicholson
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie H HuangMichael L Dustin
Jun 26, 2007·Nature·Daniel A KosterNynke H Dekker
Mar 5, 2008·Circulation·Lisa M Sullivan
Jun 28, 2008·Lab on a Chip·Javeed Shaikh MohammedDavid T Eddington
Feb 20, 1990·Biotechnology and Bioengineering·M T Tyn, T W Gusek
Oct 17, 2008·Physiological Reviews·Eva Syková, Charles Nicholson
Nov 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Delai ChenRustem F Ismagilov
Dec 18, 2008·The European Journal of Neuroscience·Jean-Marc Fritschy
Jan 9, 2009·Journal of Proteome Research·Brian M BalgleyCheng S Lee
Feb 20, 2009·IEEE Transactions on Bio-medical Engineering·Andrew A SharpKen Gall
Mar 6, 2009·Journal of Cell Science·Melanie M FrigaultClaire M Brown
Mar 21, 2009·Nature Reviews. Neuroscience·Daniel L ChaoKang Shen
Apr 3, 2009·Current Protocols in Neuroscience·Vladimir I CheferToni S Shippenberg
Aug 1, 2009·Nature Reviews. Immunology·Scott N Mueller, Ronald N Germain
Oct 16, 2009·Current Proteomics·Beth Ann ReimelTeresa A Brentnall
Dec 10, 2009·The Journal of Biological Chemistry·Yong LuoArturo Casadevall
Jan 23, 2010·MAbs·Aaron L Nelson
Sep 8, 2010·Biophysical Journal·Triantafyllos StylianopoulosRakesh K Jain
Nov 12, 2010·Annals of the Rheumatic Diseases·John D Isaacs, Gianfranco Ferraccioli
Jan 22, 2011·Journal of Cellular and Molecular Medicine·Aude CarreauClaudine Kieda
Dec 1, 2011·Analytical Chemistry·N Scott LynnDavid S Dandy
Mar 29, 2012·Bioanalysis·Kerri M Smith, Yan Xu

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