Spatially-resolved metabolic profiling of living Drosophila in neurodegenerative conditions using 1H magic angle spinning NMR.

Scientific Reports
Maxime YonSerge Birman

Abstract

Drosophila flies are versatile animal models for the study of gene mutations in neuronal pathologies. Their small size allows performing in vivo Magic Angle Spinning (MAS) experiments to obtain high-resolution 1H nuclear magnetic resonance (NMR) spectra. Here, we use spatially-resolved 1H high-resolution MAS NMR to investigate in vivo metabolite contents in different segments of the fly body. A comparative study of metabolic changes was performed for three neurodegenerative disorders: two cell-specific neuronal and glial models of Huntington disease (HD) and a model of glutamate excitotoxicity. It is shown that these pathologies are characterized by specific and sometimes anatomically localized variations in metabolite concentrations. In two cases, the modifications of 1H MAS NMR spectra localized in fly heads were significant enough to allow the creation of a predictive model.

References

Jan 1, 1992·Canadian Journal of Physiology and Pharmacology·J Edmond
Jan 1, 1985·Progress in Lipid Research·P S Sastry
Jul 20, 1957·The Journal of General Physiology·G R WYATT, G F KALE
Jun 6, 1959·Nature·D J CANDY, B A KILBY
Dec 18, 2003·NMR in Biomedicine·Claudia Zwingmann, Dieter Leibfritz
Aug 10, 2004·The Journal of Experimental Biology·Qiaofang Chen, Gabriel G Haddad
Dec 22, 2006·The Journal of Biological Chemistry·Sovan SarkarDavid C Rubinsztein
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Sep 24, 2009·Metabolomics : Official Journal of the Metabolomic Society·Ahmet T BaykalHong Li
Jun 5, 2010·CNS & Neurological Disorders Drug Targets·Frank Hirth
Jun 12, 2010·Nature Protocols·Olaf BeckonertJeremy K Nicholson
Jun 23, 2010·Human Molecular Genetics·Marie-Thérèse BessonJean-Charles Liévens
Mar 2, 2011·PloS One·Rie DanjoRichard W Ordway
Jul 7, 2011·Glia·David E Featherstone
Nov 3, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lori ZacharoffJanet M Dubinsky
Sep 15, 2012·Annual Review of Genetics·M JaiswalH J Bellen
Apr 26, 2014·Nutrients·Richard J Wurtman
Mar 10, 2015·Metabolomics : Official Journal of the Metabolomic Society·Roel Van AsscheUte Roessner
Mar 12, 2015·PloS One·Marie Thérèse BessonJean-Charles Liévens
Apr 22, 2015·Scientific Reports·Vincent Sarou-KanianJean-Claude Beloeil
Aug 4, 2015·Cell Metabolism·Anne VolkenhoffStefanie Schirmeier
Oct 9, 2015·Genetics·Leeanne McGurkNancy M Bonini
Apr 14, 2016·The Journal of Comparative Neurology·Sarah E MacNameeLynne A Oland
Nov 11, 2016·Nature·Tony Wyss-Coray

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
nuclear magnetic resonance

Software Mentioned

TOSS
PLS
DA
Matlab
TOtal

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
S GarrodElaine Holmes
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Robert A WindDonald N Rommereim
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
Hong-Chang Gao, Dong-Hai Lin
© 2022 Meta ULC. All rights reserved