Spatiotemporal analysis of DNA repair using charged particle radiation

Mutation Research
Frank TobiasB Jakob

Abstract

Approaches to visualise the dynamics of the DNA lesion processing substantially contributes to the understanding of the hierarchical organisation of the DNA damage response pathways. Charged particle irradiation has recently emerged as a tool to generate discrete sites of subnuclear damage by its means of extremely localised dose deposition at low energies, thus facilitating the spatiotemporal analysis of repair events. In addition, they are of high interest for risk estimations of human space exploration (e.g. mars mission) in the high energy regime (HZE). In this short review we will give examples for the application of charged particle irradiation to study spatiotemporal aspects of DNA damage recognition and repair in the context of recent achievements in this field. Beamline microscopy allows determining the exact kinetics of repair-related proteins after irradiation with different charged particles that induce different lesion densities. The classification into fast recruited proteins like DNA-PK or XRCC1 or slower recruited ones like 53BP1 or MDC1 helps to establish the hierarchical organisation of damage recognition and subsequent repair events. Additionally, motional analysis of DNA lesions induced by traversing particl...Continue Reading

Citations

Mar 5, 2013·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Eugene Surdutovich, A V Solov'yov
Apr 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aroumougame AsaithambyDavid J Chen
Jul 16, 2011·The Journal of Biological Chemistry·Magtouf GateiMartin F Lavin
Jul 5, 2013·PloS One·Laurin Lengert, Barbara Drossel
May 3, 2011·Cellular and Molecular Life Sciences : CMLS·Shahzad BhattiKum Kum Khanna
May 27, 2015·International Journal of Molecular Sciences·Razmik MirzayansDavid Murray
Jul 27, 2011·Biochemical and Biophysical Research Communications·Manabu KoikeAki Koike
Apr 15, 2011·Current Opinion in Cell Biology·Alexander R Ball, Kyoko Yokomori
Sep 22, 2011·International Journal of Cancer. Journal International Du Cancer·Ryuichi Okayasu
Feb 15, 2011·The EMBO Journal·Atsushi ShibataPenny A Jeggo
Feb 16, 2012·Radiation Research·B Jakob, M Durante
Mar 3, 2012·Radiation Research·Harish ShankaranMarianne B Sowa
Aug 11, 2017·International Journal of Radiation Biology·Jinhua Wu, Tom K Hei
Feb 1, 2013·International Journal of Radiation Biology·Yoshihiro FujiiTakamitsu A Kato
Jul 1, 2018·International Journal of Particle Therapy·Christine E HellwegChrista Baumstark-Khan
Feb 20, 2013·Journal of Biophotonics·Martin TomasElisa Ferrando-May
Feb 7, 2021·Journal of Cancer Research and Clinical Oncology·Yanbei KuangQiang Li
Dec 21, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Eugene SurdutovichAndrey V Solov'yov

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