Spatiotemporal analysis of putative notochordal cell markers reveals CD24 and keratins 8, 18, and 19 as notochord-specific markers during early human intervertebral disc development.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Ricardo Rodrigues-PintoJudith A Hoyland

Abstract

In humans, the nucleus pulposus (NP) is composed of large vacuolated notochordal cells in the fetus but, soon after birth, becomes populated by smaller, chondrocyte-like cells. Although animal studies indicate that notochord-derived cells persist in the adult NP, the ontogeny of the adult human NP cell population is still unclear. As such, identification of unique notochordal markers is required. This study was conducted to determine the spatiotemporal expression of putative human notochordal markers to aid in the elucidation of the ontogeny of adult human NP cells. Human embryos and fetuses (3.5-18 weeks post-conception (WPC)) were microdissected to isolate the spine anlagens (notochord and somites/sclerotome). Morphology of the developing IVD was assessed using hematoxylin and eosin. Expression of keratin (KRT) 8, KRT18, KRT19, CD24, GAL3, CD55, BASP1, CTGF, T, CD90, Tie2, and E-cadherin was assessed using immunohistochemistry. KRT8, KRT18, KRT19 were uniquely expressed by notochordal cells at all spine levels at all stages studied; CD24 was expressed at all stages except 3.5 WPC. While GAL3, CD55, BASP1, CTGF, and T were expressed by notochordal cells at specific stages, they were also co-expressed by sclerotomal cells. CD90...Continue Reading

References

May 1, 1995·Cell and Tissue Research·W GötzR Herken
Apr 20, 2002·Virchows Archiv : an International Journal of Pathology·Kanji MoriHidetoshi Okabe
Nov 18, 2005·Biochemical and Biophysical Research Communications·Nobuyuki FujitaToshio Suda
Sep 6, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Cynthia R LeeSibylle Grad
Nov 13, 2008·Journal of Cell Communication and Signaling·W Mark Erwin
Nov 28, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kyung-Suk ChoiBrian D Harfe
Feb 28, 2009·Spine·Chitra Lekha DahiaChristopher Wylie
Mar 26, 2010·Kidney International·Larissa IvanovaDouglas G Matsell
Apr 8, 2010·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Christoph WeilerNorbert Boos
Apr 13, 2010·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·J P H J RutgesW J A Dhert
Jun 23, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Makarand V RisbudIrving M Shapiro
Aug 7, 2010·Methods in Molecular Biology·Tibor KrenacsBela Molnar
Mar 3, 2011·Stem Cells and Development·Qiong QiuGretchen J Darlington
Oct 27, 2011·Disease Models & Mechanisms·Matthew R McCannCheryle A Séguin
Feb 12, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Cindy ShuJames Melrose
Jun 27, 2013·International Journal of Medical Sciences·Zhen SunZhuo-Jing Luo
Nov 29, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hiroaki HirataKotaro Nishida
Dec 7, 2013·Tissue Engineering. Part C, Methods·Sandra SpillekomMarianna A Tryfonidou
Apr 30, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Ricardo Rodrigues-PintoJudith A Hoyland
Jun 29, 2014·Arthritis Research & Therapy·Guus G H van den AkkerJan Willem Voncken
Nov 21, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Makarand V RisbudJudith A Hoyland
Dec 6, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Taryn SaggeseSusan R McGlashan
Dec 17, 2014·BMC Musculoskeletal Disorders·Benjamin GantenbeinSamantha C W Chan
Feb 1, 2015·Neuromodulation : Journal of the International Neuromodulation Society

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Citations

Jun 2, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nobuyuki FujitaMorio Matsumoto
Jul 25, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Shu-Hua YangKai-Chiang Yang
Aug 30, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·James C IatridisMakarand V Risbud
Jan 29, 2019·JOR Spine·Cheryle A SéguinZulma Gazit
Jan 2, 2019·Journal of Biological Engineering·Zhuochao LiuXingkai Zhang
May 9, 2019·Journal of Tissue Engineering and Regenerative Medicine·Tom HodgkinsonStephen M Richardson
Aug 30, 2019·JOR Spine·Matthew D HumphreysJudith A Hoyland
Oct 14, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yutaro KandaKenichiro Kakutani
Dec 19, 2020·Osteoarthritis and Cartilage·Jingcheng WangYueming Song
Nov 2, 2019·Ageing Research Reviews·Sidong YangWenyuan Ding
Jun 3, 2021·International Journal of Molecular Sciences·Martina CalióFabian Ille
Jul 18, 2021·Experimental & Molecular Medicine·Yu SongCao Yang

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