Spatiotemporal assessment of fetal bovine osteoblast culture differentiation indicates a role for BSP in promoting differentiation

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
L F CooperS W Whitson

Abstract

Fetal bovine mandible-derived osteoblasts were cultured for the purpose of obtaining a spatiotemporal assessment of bone matrix protein expression during in vitro differentiation. The results obtained from electron microscopic, immunohistological, biochemical, and molecular biological analyses indicated that these primary cultured osteoblasts produce an abundant extracellular matrix which mineralizes during a 14-day culture period. During this process, a restricted, spatiotemporal pattern of bone sialoprotein expression was indicated by immunohistological and molecular evaluations. To test the possibility that bone sialoprotein promoted the continued morphodifferentiation of osteoblastic cells, cultures were grown in the presence of anti-bone sialoprotein antibodies known to interfere with cell-bone sialoprotein attachment. Compared with cultures grown in the presence of normal rabbit serum (1:150), cultures grown in the media containing anti-bone sialoprotein antibody (1:150) failed to mineralize as demonstrated by von Kossa staining and failed to express osteocalcin and osteopontin as shown by the reverse transcription polymerase chain reaction. These results contribute to the growing evidence that bone sialoprotein is an imp...Continue Reading

References

Jun 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L D QuarlesR J Wenstrup
Aug 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J ChenJ Sodek
Jul 11, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S W WhitsonM C Falk
Jul 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K IbarakiM F Young
Oct 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G S SteinT A Owen
Apr 11, 1990·Nucleic Acids Research·M C KieferP J Barr
Sep 1, 1988·The American Journal of Anatomy·S C Marks, S N Popoff
Apr 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W J Grzesik, P G Robey
May 1, 1995·Calcified Tissue International·H Y ZhouY Kuboki
Apr 21, 1995·Annals of the New York Academy of Sciences·A L Boskey
Feb 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K L YaoJ Sodek
Aug 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K P MintzL W Fisher
Nov 1, 1993·Matrix : Collagen and Related Research·H S ShapiroJ Sodek
Jun 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C Q Lin, M J Bissell
Feb 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P BiancoP G Robey
Feb 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P BiancoP G Robey
Aug 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N AraiT Amagasa

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Citations

May 7, 2008·The Journal of Experimental Medicine·Luc MalavalJane E Aubin
Sep 20, 2008·Chemical Reviews·Adele L Boskey, Rani Roy
Jun 13, 2009·Biochemistry·Paul CurtinErdjan Salih
Sep 14, 2010·The Journal of Biological Chemistry·Hai-Yan ZhouMelvin J Glimcher
Aug 19, 2006·Dental Materials Journal·Takuya MatsumotoJunzo Takahashi
Aug 24, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J IlvesaroJ Tuukkanen
Sep 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y KatoL F Bonewald
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S L ChengL V Avioli
Feb 18, 2010·Journal of Materials Science. Materials in Medicine·M HautamäkiP K Vallittu
Sep 14, 2006·Calcified Tissue International·Jinxi WangMelvin J Glimcher
Jun 16, 2012·The Journal of Endocrinology·Katherine A StainesColin Farquharson
May 12, 2006·Periodontology 2000·Janet Moradian-OldakClark M Stanford
Aug 14, 2001·Journal of Dental Research·G B SchneiderC Stanford
Mar 28, 2001·Journal of Dental Research·L F CooperS Kadiyala
Jul 13, 2016·Journal of Tissue Engineering and Regenerative Medicine·Fen LiuFa-Ming Chen
Oct 31, 2001·The Journal of Biological Chemistry·Fan YeungLeland W K Chung
Nov 26, 2005·Clinical Oral Implants Research·Chihiro MasakiClark Stanford
Nov 20, 2015·Biological Research·Md Abdullah Al MamunMd Abdul Alim Al-Bari
Jul 12, 2017·Phytotherapy Research : PTR·Chia-Feng WuJen-Shinn Lin
Dec 19, 2009·The International Journal of Artificial Organs·Paolo GiannoniRoberto Chiesa
Oct 18, 2000·Journal of Biomedical Materials Research·A Rezania, K E Healy
Jan 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H PerinpanayagamG Schneider

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