Spatiotemporal Changes of Cerebral Monocarboxylate Transporter 8 Expression.

Thyroid : Official Journal of the American Thyroid Association
Nina-Maria WilpertHeike Biebermann

Abstract

Background: Mutations of monocarboxylate transporter 8 (MCT8), a thyroid hormone (TH)-specific transmembrane transporter, cause a severe neurodevelopmental disorder, the Allan-Herndon-Dudley syndrome. In MCT8 deficiency, TH is not able to reach those areas of the brain where TH uptake depends on MCT8. Currently, therapeutic options for MCT8-deficient patients are missing, as TH treatment is not successful in improving neurological deficits. Available data on MCT8 protein and transcript levels indicate complex expression patterns in neural tissue depending on species, brain region, sex, and age. However, information on human MCT8 expression is still scattered and additional efforts are needed to map sites of MCT8 expression in neurovascular units and neural tissue. This is of importance because new therapeutic strategies for this disease are urgently needed. Methods: To identify regions and time windows of MCT8 expression, we used highly specific antibodies against MCT8 to perform immunofluorescence labeling of postnatal murine brains, adult human brain tissue, and human cerebral organoids. Results: Qualitative and quantitative analyses of murine brain samples revealed stable levels of MCT8 protein expression in endothelial cell...Continue Reading

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Citations

Sep 29, 2020·Frontiers in Neuroscience·Pieter VancampSylvie Remaud
May 16, 2020·Frontiers in Neuroscience·Carmen Grijota-MartínezAna Guadaño-Ferraz
Jun 2, 2020·Frontiers in Endocrinology·Pieter VancampSylvie Remaud
Oct 31, 2020·Journal of the Endocrine Society·Cícera P MarcelinoAntonio C Bianco
Aug 10, 2021·Endocrinology and Metabolism·Sumiyasu IshiiNoriyuki Koibuchi

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Methods Mentioned

BETA
phosphotransferase-2
confocal microscopy

Software Mentioned

ImageJ
ProgRes C3 Capture Pro
ARRIVE
Fluoview
CellSens
GraphPad Prism

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